American Research Products

protein

RheB, 1-181aa, Human,T7-tagged, Recombinant, E.coli

01-2005

20 ug

159.00 USD

01-2005

RheB, 1-181aa, Human,T7-tagged, Recombinant, E.coli

Recombinant Protein

20 ug

159.00 USD

Ras homolog enriched in brain (RheB) is a member of the Ras superfamily that was originally identified as an immediate-early gene in brain but is also widely expressed in other tissues. RheB encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. RheB is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. Recombinant human RheB protein, fused to T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

> 95% by SDS - PAGE

Liquid in 20mM Tris pH 8.0, 1mM DTT, 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Yamagata K., et al. (1994) J Biol Chem. 269 (23) 16333-9.Tabancay AP Jr., et al. (2003). J Biol Chem. 278(41):39921-30.