American Research Products

protein

Ferric uptake regulator(FUR), 1-148 aa E.coli, Recombinant, E.coli

01-2001

10 ug

149.00 USD

01-2001

Ferric uptake regulator(FUR), 1-148 aa E.coli, Recombinant, E.coli

Recombinant Protein

10 ug

149.00 USD

Fur (ferric uptake regulator) protein is a DNA-binding protein which regulates iron-responsive genes. Fur is a small, 17-kDa, global transcriptional repressor that in the presence of iron regulates functions as diverse as iron acquisition, oxidative stress, and virulence. In Escherichia coli, members of the Fur family regulate the expression of more than 100 genes that function in processes as varied as the biosynthesis and transport of siderophores, the expression of virulence factors, the alleviation of oxidative and NO-induced stress, and the inhibition of ferritin production through the expression of RyhB. Recombinant FUR was expressed in E.coli and purified by using conventional chromatography techniques.

> 95% by SDS - PAGE

Liquid in 20mM Tris pH 8.0, 2mM CaCl2, 100mM NaCl

1 mg/ml (determined by Bradford assay)

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

O'Sullivan DJ., et al. (1994). FEMS Microbiol Lett. Apr 15; 117(3):327-32Mills SA., et al. (2005) Biochemistry. Oct 18; 44(41):13553-9