American Research Products

protein

Luciferase, 1-550aa, Firefly, His tag, E.coli, Recombinant

01-1997

20 ug

179.00 USD

01-1997

Luciferase, 1-550aa, Firefly, His tag, E.coli, Recombinant

Recombinant Protein

20 ug

179.00 USD

Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP, produces green light with a wavelength of 562 nm. Luciferase from firefly is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. Recombinant firefly Luciferase, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

> 90 by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

1mg/ml (determined by Bradford assay)

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Moore S A., et al. (1995) J Mol Biol. 249(1):195-214Gould S J., et al. (1988) Anal Biochem. 175(1):5-13.