American Research Products

protein

PTS, 1-145aa Human, His tag, E.coli, Recombinant

01-1761

20 ug

159.00 USD

01-1761

PTS, 1-145aa Human, His tag, E.coli, Recombinant

Recombinant Protein

20 ug

159.00 USD

6-Pyruvoyltetrahydropterin synthase (PTS), also known as PTPS, belongs to the family of lyases, specifically those carbon-oxygen lyases acting on phosphates. The enzyme encoded by this gene catalyzes the elimination of inorganic triphosphate from dihydroneopterin triphosphate, which is the second and irreversible step in the biosynthesis of tetrahydrobiopterin from GTP. Tetrahydrobiopterin, also known as BH(4), is an essential cofactor and regulator of various enzyme activities, including enzymes involved in serotonin biosynthesis and NO synthase activity. Mutations in this gene result in hyperphenylalaninemia. Recombinant human PTS, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

> 90% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 20% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Thony B., et al. (1992) Biochem Biophys Res Commun. 189:1437-43.Milstien S., et al. (1989) J Biol Chem. 264(14):8066-73.