protein

Thymidylate synthase, 1-313aa, Human, His tag, E.coli, Recombinant

01-1756

20 ug

159.00 USD

Thymidylate synthase, 1-313aa, Human, His tag, E.coli, Recombinant

Recombinant Protein

20 ug

159.00 USD

Thymidylate synthase is an intracellular enzyme critical for de novo synthesis of DNA. This function maintains the dTMP(thymidine-5-prime monophosphate) pool critical for DNA replication and repair. In cancer, expression of this protein is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-FU). Resistance or lack of response to 5-FU is attributed to the elevation of thymidylate synthase activity. Recombinant human Thymidylate synthase protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

> 95% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1 M NaCl.

1 mg/ml (determined by Bradford assay)

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Chiu TH., et al. (2009) Anticancer Res. 29(11):4503-11.Peï¾–a MM., et al. (2009) J Biol Chem. 284(46):31597-607.