American Research Products

protein

FEN1, 1-380aa, Human, E.coli, Recombinant

01-1753-1

10 ug

189.00 USD

01-1753-1

FEN1, 1-380aa, Human, E.coli, Recombinant

Recombinant Protein

10 ug

189.00 USD

FEN-1, also known as Flap structure-specific endonuclease 1, removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. FEN-1 is highly homologous to yeast Rad2. The C-terminal region of FEN-1 may bind to PCNA, thus allowing FEN-1 to function as an exonuclease in DNA replication. Recombinant human FEN-1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

> 90% by SDS - PAGE

Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Hakoyama T., et al. (2009) Nature. 462(7272):514-7.Lal A., et al. (2009) Mol Cell. 35(5):610-25.