American Research Products

protein

ACADS, 25-412aa, Human, His tag, E.coli, Recombinant

01-1752-S

10 ug

209.00 USD

01-1752-S

ACADS, 25-412aa, Human, His tag, E.coli, Recombinant

Recombinant Protein

10 ug

209.00 USD

ACADS (Acyl-Coenzyme A dehydrogenase), also known as SCAD or ACAD3, is a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations of ACADS have been associated with fatty acid oxidation defects and metabolic diseases such as short-chain acyl-CoA dehydrogenase deficiency (SCAD deficiency). Recombinant human ACADS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

> 95% by SDS - PAGE

Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT 0.1 M Nacl, and 20% glycerol

0.5 mg/ml (determined by Bradford assay)

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

McAndrew RP., et al. (2008) J Biol Chem. 283(14):9435-43.Tafti M., et al. (2003) Nat Genet. 34(3):320-5.