American Research Products

protein

GAMT, 1-236aa, Human, His tag, E.coli, Recombinant

01-1743

20 ug

129.00 USD

01-1743

GAMT, 1-236aa, Human, His tag, E.coli, Recombinant

Recombinant Protein

20 ug

129.00 USD

Guanidinoacetate N-methyltransferase isoform a, also known as GAMT is a methyltansferase that converts guanidoacetate to creatine, using S-adenosylmethionine as the methyl domor. This enzyme participates in the two-step synthesis of the compound creatine from the protein building blocks glycine, arginine, and methionine. It is involved in providing energy for muscle contraction, and is also important in nervous system functioning. Also GAMT is active in the liver, pancreas, and kidneys. Recombinant human GAMT, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

> 95% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 1mM DTT,10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Almeida LS., et al. (2007) Mol Genet Metab. 91(1):1-6.Jenne DE., et al. (1997) Biochem Biophys Res Commun. 238(3):723-7.