Anti-SCN2B (NaVβ2) Antibody | Alomone Labs asc-007 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SCN2B (NaVβ2) Antibody

catalog :

asc-007

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Jo Y, Lee J, Lee S, Kwon I, Cho H. Poly-dipeptides produced from C9orf72 hexanucleotide repeats cause selective motor neuron hyperexcitability in ALS. Proc Natl Acad Sci U S A. 2022;119:e2113813119 pubmed publisher
Gwanyanya A, Andriulė I, Istrate B, Easmin F, Mubagwa K, Macianskiene R. Modulation of the Cardiac Myocyte Action Potential by the Magnesium-Sensitive TRPM6 and TRPM7-like Current. Int J Mol Sci. 2021;22: pubmed publisher
Hu T, Li S, Liang W, Li S, Lu M, Chen B, et al. Notoginsenoside R1-Induced Neuronal Repair in Models of Alzheimer Disease Is Associated With an Alteration in Neuronal Hyperexcitability, Which Is Regulated by Nav. Front Cell Neurosci. 2020;14:280 pubmed publisher
Cortada E, Brugada R, Verges M. N-Glycosylation of the voltage-gated sodium channel β2 subunit is required for efficient trafficking of NaV1.5/β2 to the plasma membrane. J Biol Chem. 2019;294:16123-16140 pubmed publisher
Murenzi E, Toltin A, Symington S, Morgan M, Clark J. Evaluation of microtransplantation of rat brain neurolemma into Xenopus laevis oocytes as a technique to study the effect of neurotoxicants on endogenous voltage-sensitive ion channels. Neurotoxicology. 2017;60:260-273 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes under reducing (lanes 1 and 2) or non-reducing (lanes 3 and 4) conditions:* - 1,3. Anti-SCN2B (NaVβ2) Antibody (#ASC-007), (1:200).2,4. Anti-SCN2B (NaVβ2) Antibody, preincubated with SCN2B/Navβ2 Blocking Peptide (#BLP-SC007). * NaVβ2 is disulfide-linked to the α subunit of voltage-gated Na+ channels (Schmidt, J.W. and Catterall, W.A. (1986) Cell 46, 437.)

image 2 :

Expression of NaVβ2 in mouse cerebellum - Immunohistochemical staining of mouse cerebellum using Anti-SCN2B (NaVβ2) Antibody (#ASC-007). A. NaVβ2 channel (red) appears in Purkinje cells (vertical arrows) and in small cells in the molecular layer (horizontal arrows, Mol). B. Staining of Purkinje nerve cells with mouse anti-calcium binding protein (CBD28k, green) demonstrates the restriction of NaVβ2 to cell bodies. C. Confocal merge of NaVβ2 and CBD28k.

image 3 :

Expression of NaV?2in rat cerebellum - Immunohistochemical staining of rat cerebellum usingAnti-SCN2B (NaV?2)Antibody (#ASC-007). A. NaV?2channel (red) appears in Purkinje cells (horizontal arrows) and is distributed diffusely in the molecular layer (Mol). B. Staining of Purkinje nerve cells with mouse anti-calcium binding protein (CBD28k green) demonstrates the restriction of NaV?2to cell bodies. C. Confocal merge of NaV?2and CBD28k.

product information

CAT :

ASC-007

SKU :

ASC-007-CF_0.2 ml

Product Name :

Anti-SCN2B (NaVβ2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P54900

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC007

Homology :

Human - 17/19 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KTEEEGKTDGEGNAEDGAK, corresponding to amino acid residues 197-215 of rat β2 subunit of voltage-gated Na+ channels

Product Page - Scientific background :

Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.2,3 Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β-subunits (NaVβ-subunit).4The NaV β-subunit gene family consists of four members: β1 (SCN1B), β2 (SCN2B), β3 (SCN3B), and β4 (SCN4B) having type I topology, containing an extracellular amino-terminus, a single transmembrane segment, and an intracellular carboxyl-terminus. They modulate channel gating, assembly, and cell surface expression in heterologous cell systems.5NaV β-subunits are cell adhesion molecules of the Ig superfamily, which interact with extracellular matrix, transmembrane signaling, and cell adhesion molecules.5In the adult central nervous system and heart, sodium channels are associated with β1- β4 subunits, whereas in adult skeletal muscle they are associated only with the β1 subunit.4,5 This association appears to be a late event in sodium channel biosynthesis.6Expression of β2-subunits increases in sensory neurons following nerve injury. β2 deficient mice develop less mechanical allodynia in comparison to wild type mice, suggesting a role for β2 subunit in pain sensation.7

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Voltage-gated sodium channel subunit beta-2

Short Description :

A Rabbit Polyclonal Antibody to NaVβ2 Subunit

Long Description :

Anti-SCN2B (NaVβ2) Antibody (#ASC-007) is a highly specific antibody directed an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NaVβ2 subunit from human, rat, and mouse samples.

Negative Control :

BLP-SC007

Positive Control :

Synonyms :

Voltage-gated sodium channel subunit beta-2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN2B

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage