Anti-SCN1A (NaV1.1) Antibody | Alomone Labs asc-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SCN1A (NaV1.1) Antibody

catalog :

asc-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

more info or order :

Alomone Labs product webpage

citations: 23

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; 1:500; fig 8	Alshammari M, Alshammari T, Laezza F. Improved Methods for Fluorescence Microscopy Detection of Macromolecules at the Axon Initial Segment. Front Cell Neurosci. 2016;10:5 pubmed publisher
	Teunissen M, Lewerissa E, van Hugte E, Wang S, Ockeloen C, Koolen D, et al. ANK2 loss-of-function variants are associated with epilepsy, and lead to impaired axon initial segment plasticity and hyperactive network activity in hiPSC-derived neuronal networks. Hum Mol Genet. 2023;32:2373-2385 pubmed publisher
	Voskobiynyk Y, Battu G, Felker S, Cochran J, Newton M, Lambert L, et al. Aberrant regulation of a poison exon caused by a non-coding variant in a mouse model of Scn1a-associated epileptic encephalopathy. PLoS Genet. 2021;17:e1009195 pubmed publisher
	Jansen N, Perez C, Schenke M, van Beurden A, Dehghani A, Voskuyl R, et al. Impaired θ-γ Coupling Indicates Inhibitory Dysfunction and Seizure Risk in a Dravet Syndrome Mouse Model. J Neurosci. 2021;41:524-537 pubmed publisher
	Chen X, Zhang J, Song Y, Yang P, Yang Y, Huang Z, et al. Deficiency of anti-inflammatory cytokine IL-4 leads to neural hyperexcitability and aggravates cerebral ischemia-reperfusion injury. Acta Pharm Sin B. 2020;10:1634-1645 pubmed publisher
	Lim K, Han Z, Jeon H, Kach J, Jing E, Weyn Vanhentenryck S, et al. Antisense oligonucleotide modulation of non-productive alternative splicing upregulates gene expression. Nat Commun. 2020;11:3501 pubmed publisher
	Housley S, Nardelli P, Carrasco D, Rotterman T, Pfahl E, Matyunina L, et al. Cancer Exacerbates Chemotherapy-Induced Sensory Neuropathy. Cancer Res. 2020;80:2940-2955 pubmed publisher
	Tan Y, Liu G, Chen H, Lu M, Chen B, Hu T, et al. Exercise-Induced Cognitive Improvement Is Associated with Sodium Channel-Mediated Excitability in APP/PS1 Mice. Neural Plast. 2020;2020:9132720 pubmed publisher
	Jansen N, Dehghani A, Breukel C, Tolner E, van den Maagdenberg A. Focal and generalized seizure activity after local hippocampal or cortical ablation of NaV 1.1 channels in mice. Epilepsia. 2020;61:e30-e36 pubmed publisher
	Jansen N, Dehghani A, Linssen M, Breukel C, Tolner E, van den Maagdenberg A. First FHM3 mouse model shows spontaneous cortical spreading depolarizations. Ann Clin Transl Neurol. 2020;7:132-138 pubmed publisher
	Xie Y, Ng N, Safrina O, Ramos C, Ess K, Schwartz P, et al. Comparisons of dual isogenic human iPSC pairs identify functional alterations directly caused by an epilepsy associated SCN1A mutation. Neurobiol Dis. 2020;134:104627 pubmed publisher
	Ricobaraza A, Mora Jimenez L, Puerta E, Sanchez Carpintero R, Mingorance A, Artieda J, et al. Epilepsy and neuropsychiatric comorbidities in mice carrying a recurrent Dravet syndrome SCN1A missense mutation. Sci Rep. 2019;9:14172 pubmed publisher
	Ciccone R, Franco C, Piccialli I, Boscia F, Casamassa A, de Rosa V, et al. Amyloid β-Induced Upregulation of Nav1.6 Underlies Neuronal Hyperactivity in Tg2576 Alzheimer's Disease Mouse Model. Sci Rep. 2019;9:13592 pubmed publisher
	Angus M, Peters C, Poburko D, Brimble E, Spelbrink E, Ruben P. Case studies in neuroscience: a novel amino acid duplication in the NH2-terminus of the brain sodium channel NaV1.1 underlying Dravet syndrome. J Neurophysiol. 2019;122:1975-1980 pubmed publisher
	Johansson J, Karema Jokinen V, Hakanen S, Jylhä A, Uusitalo H, Vihinen Ranta M, et al. Sodium channels enable fast electrical signaling and regulate phagocytosis in the retinal pigment epithelium. BMC Biol. 2019;17:63 pubmed publisher
	Nissenkorn A, Almog Y, Adler I, Safrin M, Brusel M, Marom M, et al. In vivo, in vitro and in silico correlations of four de novo SCN1A missense mutations. PLoS ONE. 2019;14:e0211901 pubmed publisher
	Koleske M, Bonilla I, Thomas J, Zaman N, Baine S, Knollmann B, et al. Tetrodotoxin-sensitive Navs contribute to early and delayed afterdepolarizations in long QT arrhythmia models. J Gen Physiol. 2018;150:991-1002 pubmed publisher
	Guan G, Zhao M, Xu X, Boczek T, Mao X, Li Z, et al. Abnormal changes in voltage-gated sodium channels subtypes NaV1.1, NaV1.2, NaV1.3, NaV1.6 and CaM/CaMKII pathway in low-grade astrocytoma. Neurosci Lett. 2018;674:148-155 pubmed publisher
	Martinez Losa M, Tracy T, Ma K, Verret L, Clemente Perez A, Khan A, et al. Nav1.1-Overexpressing Interneuron Transplants Restore Brain Rhythms and Cognition in a Mouse Model of Alzheimer's Disease. Neuron. 2018;98:75-89.e5 pubmed publisher
	Hu T, Xiao Z, Mao R, Chen B, Lu M, Tong J, et al. Nav?2 knockdown improves cognition in APP/PS1 mice by partially inhibiting seizures and APP amyloid processing. Oncotarget. 2017;8:99284-99295 pubmed publisher
	Johnson K, Herold K, Milner T, Hemmings H, Platholi J. Sodium channel subtypes are differentially localized to pre- and post-synaptic sites in rat hippocampus. J Comp Neurol. 2017;525:3563-3578 pubmed publisher
	Carrasco D, Vincent J, Cope T. Distribution of TTX-sensitive voltage-gated sodium channels in primary sensory endings of mammalian muscle spindles. J Neurophysiol. 2017;117:1690-1701 pubmed publisher
	Murenzi E, Toltin A, Symington S, Morgan M, Clark J. Evaluation of microtransplantation of rat brain neurolemma into Xenopus laevis oocytes as a technique to study the effect of neurotoxicants on endogenous voltage-sensitive ion channels. Neurotoxicology. 2017;60:260-273 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-SCN1A (NaV1.1) Antibody (#ASC-001), (1:200).2. Anti-SCN1A (NaV1.1) Antibody, preincubated with SCN1A/Nav1.1 Blocking Peptide (#BLP-SC001).

image 2 :

Expression of NaV1.1 sodium channel in mouse cerebellum - Immunohistochemical staining of mouse cerebellum using Anti-SCN1A (NaV1.1) Antibody (#ASC-001). A. The distribution of NaV1.1 (red) forms a band (arrows) in the molecular layer (Mol), close to the Purkinje cell bodies. B. Purkinje nerve cells are stained with mouse anti-Parvalbumin (green). C. Confocal merge of NaV1.1 and Parvalbumin.

product information

CAT :

ASC-001

SKU :

ASC-001-CF_0.2 ml

Product Name :

Anti-SCN1A (NaV1.1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P04774

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-SC001

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular loop between domains I and II

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)TASEHSREPSAAGRLSD, corresponding to amino acid residues 465-481 of rat NaV1.1

Product Page - Scientific background :

Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability1. NaV channels are activated in response to depolarization and selectively allow the flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.94-5. The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9)2-3.Mammalian sodium channels are heterotrimers composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Na+ channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas Na+ channels in adult skeletal muscle have only the β1 subunit6,7.NaV1.1, also referred to as SCN1A, is a tetrodotoxin-sensitive channel and is broadly expressed in neurons7.Mutations in NaV1.1 are associated with at least two forms of epilepsy. Gain-of-function missense mutations are a primary cause of generalized epilepsy with febrile seizures plus (GEFS+). Loss-of-function mutations cause severe myoclonic epilepsy of infancy (SMEI)8,9.

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

Brain type I sodium channel, BI, Sodium channel protein type 1 subunit α

Short Description :

A Rabbit Polyclonal Antibody to SCN1A (NaV1.1) Channel

Long Description :

Anti-SCN1A (NaV1.1) Antibody (#ASC-001) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize NaV1.1 from rat, human, and mouse samples.

Negative Control :

BLP-SC001

Positive Control :

Synonyms :

Brain type I sodium channel, BI, Sodium channel protein type 1 subunit α

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SCN1A

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage