Anti-NCX1 (SLC8A1) Antibody | Alomone Labs anx-011 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NCX1 (SLC8A1) Antibody

catalog :

anx-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; fig 4	Ge W, Yuan M, Ceylan A, Wang X, Ren J. Mitochondrial aldehyde dehydrogenase protects against doxorubicin cardiotoxicity through a transient receptor potential channel vanilloid 1-mediated mechanism. Biochim Biophys Acta. 2016;1862:622-634 pubmed publisher
	Garcia Elias A, Tajes M, Ya xf1 ez Bisbe L, Enjuanes C, Com xed n Colet J, Serra S, et al. Atrial Fibrillation in Heart Failure Is Associated with High Levels of Circulating microRNA-199a-5p and 22-5p and a Defective Regulation of Intracellular Calcium and Cell-to-Cell Communication. Int J Mol Sci. 2021;22: pubmed publisher
	Xie D, Geng L, Xiong K, Zhao T, Wang S, Xue J, et al. Cold-Inducible RNA-Binding Protein Prevents an Excessive Heart Rate Response to Stress by Targeting Phosphodiesterase. Circ Res. 2020;126:1706-1720 pubmed publisher
	Bragança B, Nogueira Marques S, Ferreirinha F, Fontes Sousa A, Correia de Sá P. The Ionotropic P2X4 Receptor has Unique Properties in the Heart by Mediating the Negative Chronotropic Effect of ATP While Increasing the Ventricular Inotropy. Front Pharmacol. 2019;10:1103 pubmed publisher
	Sherkhane P, Kapfhammer J. Chronic pharmacological blockade of the Na+ /Ca2+ exchanger modulates the growth and development of the Purkinje cell dendritic arbor in mouse cerebellar slice cultures. Eur J Neurosci. 2017;46:2108-2120 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-NCX1 (SLC8A1) Antibody (#ANX-011), (1:200).3,4. Anti-NCX1 (SLC8A1) Antibody, preincubated with NCX1/SLC8A1 Blocking Peptide (#BLP-NX011).

image 2 :

Expression of NCX1 in rat heart - Immunohistochemical staining of rat heart paraffin-embedded sections using Anti-NCX1 (SLC8A1) Antibody (#ANX-011), (1:100). Hematoxilin is used as the counterstain. NCX1 labeling (brown) appears in the cardiac muscle cells (CM), and not in other parts of the tissue, such as the blood vessel endothelium (E), the connective tissue (CT) and smooth muscle (SM).

image 3 :

Peptide (C)EVDERDQDDEEAR corresponding to amino acid residues 308-320 of rat NCX1 (AccessionQ01728). 3rdintracellular loop.

product information

CAT :

ANX-011

SKU :

ANX-011-CF_0.2 ml

Product Name :

Anti-NCX1 (SLC8A1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q01728

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NX011

Homology :

Human, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EVDERDQDDEEAR, corresponding to amino acid residues 308-320 of rat NCX1

Product Page - Scientific background :

Ca2+ has proven to be a universal signaling molecule in excitable and non-excitable cells. However, being that its intracellular concentration is 1000 times lower than that of the extracellular milieu, it is important for the cell to keep this ratio for proper function. NCX, a Na+/Ca2+ exchanger is responsible for most of the efflux of Ca2+ from the cell1-3. The NCX transporter is a member of the SLC8 family of solute carriers which in turn belong to the CaCA superfamily1,4,5.NCX-1 is one of three Na+/Ca2+ exchangers (NCX-1, NCX-2, NCX-3) leading to one Ca2+ movement across the plasma membrane in exchange of three Na+ influx. However, the transporter can reverse the direction of the transport if the concentrations of Na+ and Ca2+ change6. The transporter has nine transmembrane domains and intracellular N- and C-terminals. Between tansmembrane domains 5 and 6, the presence of an extra-long intracellular loop, termed the f-loop, is responsible for regulating the activity of NCX-1 via several different mechanisms like ion binding, phosphorylation, etc. The f-loop also has sites which undergo alternative splicing7.Of the three NCX expressed in mammalian cells, NCX-1 is the most widely expressed. Its expression is detected in the heart, brain, and kidney. NCX-1 undergoes alternative splicing in a tissue-dependent manner. The first splice region does not change the overall structure of the protein but rather enables the expression of the gene specific to the tissues which require the expression of the gene. The second splicing site leads to a number of proteins varying in length. NCX-2 expression is much more limited; it is expressed only in neurons. NCX-3 is expressed in skeletal muscle and in some regions of the brain and undergoes alternative splicing in a similar fashion to that of NCX-11,8.Due to its central role in modulating Ca2+ levels in the cell, NCX-1 has become a pharmaceutical target in the development of drugs for various heart diseases and neurological disorders1.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Sodium/calcium exchanger 1, Na+/Ca2+ exchange protein 1

Short Description :

A Rabbit Polyclonal Antibody to Na+/Ca2+ Exchanger 1 (SLC8A1)

Long Description :

Anti-NCX1 (SLC8A1) Antibody (#ANX-011) is a highly specific antibody directed against an epitope of the rat Na+/Ca2+ exchange protein 1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NCX1 from human, rat, and mouse samples.

Negative Control :

BLP-NX011

Positive Control :

Synonyms :

Sodium/calcium exchanger 1, Na+/Ca2+ exchange protein 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC8A1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage