Anti-EAAT1 (GLAST) (extracellular) Antibody | Alomone Labs agc-021 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-EAAT1 (GLAST) (extracellular) Antibody

catalog :

agc-021

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:400; fig s1	Ruegsegger C, Stucki D, Steiner S, Angliker N, Radecke J, Keller E, et al. Impaired mTORC1-Dependent Expression of Homer-3 Influences SCA1 Pathophysiology. Neuron. 2016;89:129-46 pubmed publisher
	Stoica A, Larsen B, Assentoft M, Holm R, Holt L, Vilhardt F, et al. The ?2?2 isoform combination dominates the astrocytic Na+ /K+ -ATPase activity and is rendered nonfunctional by the ?2.G301R familial hemiplegic migraine type 2-associated mutation. Glia. 2017;65:1777-1793 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-EAAT1 (GLAST) (extracellular) Antibody (#AGC-021) (1:500).3,4. Anti-EAAT1 (GLAST) (extracellular) Antibody, preincubated with EAAT1/GLAST (extracellular) Blocking Peptide (#BLP-GC021).

image 2 :

Expression of EAAT1 in rat hippocampus - Immunohistochemical staining was performed in perfusion-fixed free floating rat brain sections (frozen) using Anti-EAAT1 (GLAST) (extracellular) Antibody (#AGC-021), (1:100). A. EAAT1 staining (green) is particularly intense along the subgranular layer (arrows). B. Staining with mouse anti glial fibrillary acidic protein (red). C. Merged picture confirms presence of densely packed astrocytes along the subgranular layer. DAPI is used as a general cell marker (blue).

image 3 :

Expression of EAAT1 in human U-87 MG cells - Cell surface detection of EAAT1 in live intact human U-87 MG glioblastoma cells. A. Extracellular staining of cells using Anti-EAAT1 (GLAST) (extracellular) Antibody (#AGC-021)(1:100) followed by goat anti-rabbit-Alexa-Fluor-594 secondary antibody (red). B. Live image of the cells. C. Merge of A and B.

product information

CAT :

AGC-021

SKU :

AGC-021-CF_0.2 ml

Product Name :

Anti-EAAT1 (GLAST) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P24942

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-GC021

Homology :

Mouse - identical; human 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KQFKTSYEKRSFK, corresponding to amino acids 188-200 of rat EAAT1

Product Page - Scientific background :

L-Glutamate (Glu) is an abundant amino acid that functions as the major excitatory neurotransmitter in the central nervous system. However, excess of Glu in the extracellular synaptic milieu leads to neuronal cell death by a process known as excitotoxicity.The extracellular levels of Glu are regulated by a family of high affinity plasma membrane transporters called excitatory amino acid transporters (EAATs) which are responsible for re-uptake of Glu into the cells.1,2 The EAAT family includes five members (EAAT1-EAAT5) that are members of the solute carrier family 1 (SLC1) of sodium-dependent transporters that also includes the neutral amino acid transporters ASCT1 and ASCT2.The Glu transporters present an unusual topology of eight transmembrane domains with two re-entrant loops and intracellular N- and C- termini. The transporter is likely assembled as a trimer where each monomer is a functional unit capable of binding the Glu substrate.The transport of Glu into the cells by the EAAT transporters is coupled to the Na+ and K+ electrochemical gradient as a driving force. Hence, the uptake of Glu is dependent on the co-transport of three Na+ and one H+ ions, and the counter transport of one K+ ion. In addition, to the well documented Glu uptake, the EAAT transporters show a Glu-independent Cl- conductance. The physiological significance of the Cl- current through the EAATs is currently unknown.1,2EAAT1 as well as EAAT2, is expressed predominantly in glia cells (hence its original name: glial glutamate transporter or GLAST), while EAAT3, EAAT4 and EAAT5 are mostly expressed in neurons.As mentioned earlier, EAAT transporters represent the only (significant) mechanism for removal of glutamate from the extracellular fluid and hence are essential for the long-term maintenance of low and non-toxic concentrations of glutamate and the preservation of normal excitatory synaptic transmission.In addition, to Glu uptake the glutamate transporters provide glutamate for the synthesis of γ-Aminobutyric acid (GABA), glutathione and protein, suggesting an interactive role between EAATs and cellular metabolism.1,2Dysregulation of EAATs activities has been implicated in several neurodegenerative disorders such as Alzheimer's disease, traumatic brain injury, epilepsy and schizophrenia, suggesting that EAATs can be a useful target for the treatment of these conditions.1,2

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Excitatory amino acid transporter 1, Sodium-dependent glutamate/aspartate transporter 1, GLAST-1, SLC1A3

Short Description :

A Rabbit Polyclonal Antibody to Excitatory Amino Acid Transporter 1 (EAAT1, GLAST)

Long Description :

Anti-EAAT1 (GLAST) (extracellular) Antibody (#AGC-021) is a highly specific antibody directed against an epitope of rat Excitatory amino acid transporter 1. The antibody can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize EAAT1 from rat, human and mouse samples.

Negative Control :

BLP-GC021

Positive Control :

Synonyms :

Excitatory amino acid transporter 1, Sodium-dependent glutamate/aspartate transporter 1, GLAST-1, SLC1A3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC1A3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage