Wortmannin | Alomone Labs W-400 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Wortmannin

catalog :

W-400

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Yu C, Micaroni M, Puyskens A, Schultz T, Yeo J, Stanley A, et al. RP105 Engages Phosphatidylinositol 3-Kinase p110Î´ To Facilitate the Trafficking and Secretion of Cytokines in Macrophages during Mycobacterial Infection. J Immunol. 2015;195:3890-900 pubmed publisher
Noss I, Ozment T, Graves B, Kruppa M, Rice P, Williams D. Cellular and molecular mechanisms of fungal β-(1→6)-glucan in macrophages. Innate Immun. 2015;21:759-69 pubmed publisher
Qiu J, Zhang C, Borgquist A, Nestor C, Smith A, Bosch M, et al. Insulin excites anorexigenic proopiomelanocortin neurons via activation of canonical transient receptor potential channels. Cell Metab. 2014;19:682-93 pubmed publisher
Qiu J, Fang Y, Rønnekleiv O, Kelly M. Leptin excites proopiomelanocortin neurons via activation of TRPC channels. J Neurosci. 2010;30:1560-5 pubmed publisher

image

image 1 :

Alomone Labs Wortmannin inhibits AKT phosphorylation via PI3-kinase in NIH-3T3 cells. - Cells were preincubated for 30 min in different concentrations ofLY 294002(#L-300) orWortmannin(#W-400) and then stimulated with PDGF (3 ng/ml) for 20 min. Cell proteins were resolved by SDS-PAGE and probed with (A) anti-phospho-AKT antibody followed by stripping and reprobed with (B) anti-Akt antibody.

product information

CAT :

W-400

SKU :

W-400_1 mg

Product Name :

Wortmannin

Group Type :

Non Antibodies

Product Type :

S. Molecules

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

PI 3 Kinase is an enzyme implicated in growth factor signal transduction by associating with receptor and non-receptory tyrosine kinases. Wortmannin, a fungal metabolite (Penicillium fumiculosum), is a potent and specific inhibitor of phosphatidylinositol 3-kinase (PI-3K).1 It is highly cell-permeable, binds to the catalytic subunit of PI-3K and inhibits the enzyme activity in vitro and in vivo.2,3In purified preparations and cell-free cytosolic fractions it is active at 5 nM. In fibroblasts Wortmannin abolishes PDGF-mediated Inositol(3,4,5) P3 formation (IC50 = 5 nM).4 In guinea pig neutrophils it inhibits fMLP induced PIP3 and superoxide anion production (IC50 = 50 nM), and also phospholipase D activation.5,6 In PC12 cells treated with NGF, Wortmannin blocks the elongation of neurites.7 In rat basophilic leukemia (RBL-2H3) cells it inhibits IgE mediated histamine secretion.8 At higher concentrations Wortmanin inhibits other kinases such as myosin light chain kinase (IC50 = 200 nM)9 and MAP kinase activation (IC50 = 200-300 nM) induced by platelet activating factor.10

Supplier :

Alomone Labs

Long Description :

Wortmannin (#W-400) is a highly pure, natural, and biologically active compound.

Short Description :

A Selective, Potent Inhibitor of PI3-Kinase

MW :

428.4

Synonyms :

SL-2052

Source :

Natural

Molecular formula :

C23H24O8.

Effective Concentration :

20-100 nM.

Activity :

Wortmannin is a potent and specific inhibitor of PI3-kinase1. In PC12 cells treated with NGF, Wortmannin blocks the elongation of neurites2. At higher concentrations Wortmanin inhibits other kinases3,4.

Reconstitution and Solubility :

DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to four weeks at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>98%

CAS No :

19545-26-7

Form :

Lyophilized Powder

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

(1S,6br,9aS,11R,11bR) 11-(Acetyloxy)-1,6b,7,8,9a,10,11,11b-octahydro-1-(metho xymethyl)-9a,11b-dimethyl-3Hfuro[4,3,2-de]indeno[4,5,- h]-2-h]-2-benzopyran-3,6,9-trione.

more info or order :

Alomone Labs product webpage