VU0529331 | Alomone Labs V-155 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

VU0529331

catalog :

V-155

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs HC-030031 inhibits TRPA1 currents induced by AITC in HEK-293 cells. - Dose-response curve ofHC-030031(#H-105) inhibition of human TRPA1-mediated increase in intracellular Ca2+. Cells were loaded with Calcium 5 dye incubated for 10 min with HC-030031 and stimulated by 1 M allyl isothiocyanate. Changes in intracellular Ca2+following stimulation were detected as changes in maximum relative fluorescence (RFU). IC50was determined at 13.8 M.

image 2 :

product information

CAT :

V-155

SKU :

V-155_10 mg

Product Name :

VU0529331

Group Type :

Non Antibodies

Product Type :

S. Molecules

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

VU0529331 is an activator of the homomeric and of non-GIRK1-containing GIRK channels. The activity of the compound is much stronger on non-GIRK1/X channels. VU0529331 does not change the potassium selectivity or rectification of GIRK channels1. VU0529331 activates GIRK2 and GIRK4 homomeric channels expressed in HEK293 cells and demonstrates EC50 values of 5.1 and 22.3 µM respectively1.The GIRK family, also known as the Kir3 family, is part of the larger inwardly rectifying potassium channel (Kir) family. GIRK channels play an important role in the regulation of cellular excitability. GIRK channels are heterotetrameric or homotetrameric combinations of the GIRK1−GIRK4 subunits. GIRK channels are associated with a variety of neurological disorders and alcohol and drug addiction1,2.

Supplier :

Alomone Labs

Target :

Kir3.2, Kir3.4 channels

Long Description :

VU0529331 (#V-155) is a highly pure, synthetic, and biologically active compound.

Short Description :

An Activator of Homomeric and Non-GIRK1-Containing GIRK Channels

MW :

384.4

Synonyms :

(2-(3,4-dimethylphenyl)-3-(1H-pyrrol-1-yl)pyrrolo[3,4-c]pyrazol-5(2H,4H,6H)-yl)(pyrazin-2-yl)methanone

Source :

Synthetic

Molecular formula :

C22H20N6O.

Effective Concentration :

5-100 µM.

Activity :

VU0529331 is a novel activator of homomeric and of non-GIRK1-containing G-protein-activated inward rectifier potassium (GIRK) channels. VU0529331 was shown to significantly activate GIRK2 and GIRK4 homomeric channels expressed in HEK293 cells (GIRK2 EC50=5.1 µM).1

Reconstitution and Solubility :

Soluble to 15 mM in DMSO with gentle warming. Centrifuge all products before handling (10000 x g 5 min).

Solubility :

Soluble to 15 mM in DMSO with gentle warming. Centrifuge all products before handling (10000 x g 5 min).

Storage of solutions :

Store at -20°C for several months. It is recommended to aliquot stock solutions to prevent repeated thawing and freezing.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>99% (HPLC)

CAS No :

1286725-49-2

Form :

Lyophilized Powder

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

[2-(3,4-dimethylphenyl)-3-pyrrol-1-yl-4,6-dihydropyrrolo[3,4-c]pyrazol-5-yl]-pyrazin-2-ylmethanone.

more info or order :

Alomone Labs product webpage