U0126 | Alomone Labs U-400 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

U0126

catalog :

U-400

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Clauzure M, Valdivieso A, Massip Copiz M, Schulman G, Teiber M, Santa Coloma T. Disruption of interleukin-1? autocrine signaling rescues complex I activity and improves ROS levels in immortalized epithelial cells with impaired cystic fibrosis transmembrane conductance regulator (CFTR) function. PLoS ONE. 2014;9:e99257 pubmed publisher
Lue H, Yang X, Wang R, Qian W, Xu R, LYLES R, et al. LIV-1 promotes prostate cancer epithelial-to-mesenchymal transition and metastasis through HB-EGF shedding and EGFR-mediated ERK signaling. PLoS ONE. 2011;6:e27720 pubmed publisher

image

image 1 :

image 2 :

Alomone Labs U0126 inhibits P42/44 MAPK phosphorylation via MEK1/MEK2 in C6 glioma cells. - Cells were grown to 70% confluence and serum starved for 1.5 h. The cells were then incubated for 2 h with various concentrations ofU0126(#U-400) and stimulated with 7 ng/ml PDGF-AA. Cell proteins were resolved by SDS-PAGE and probed with anti-phospho-P42/44 MAPK (upper panel) and with anti-P42/44 MAPK (lower panel). The phosphorylation of P42/44 decreased in a dose-dependent manner.

product information

CAT :

U-400

SKU :

U-400_1 mg

Product Name :

U0126

Group Type :

Non Antibodies

Product Type :

S. Molecules

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

U0126 is a chemically synthesized organic compound that was initially recognized as a cellular AP-1 antagonist, and found to be a very selective and highly potent inhibitor of the Mitogen-Activated Protein Kinase (MAPK) cascade by inhibiting its immediate upstream activators, Mitogen-Activated Protein Kinase Kinase 1 and 2 (also known as MEK1 and MEK2, IC50: 70 and 60 nM respectively). U0126 inhibits both active and inactive MEK1,2, unlike PD098059 which only inhibits activation of inactive MEK1,2. Blockade of MEK activation would prevent downstream phosphorylation of a number of factors including p62TCF (Elk-1), an upstream inducer of c-Fos and c-Jun, components of the AP-1 complex.1-4Inhibition of the MEK/ERK pathway by U0126 also prevents all effects of oncogenic H-Ras and K-Ras, inhibits part of the effects triggered by growth factors and blocks the production of inflammatory cytokines and matrix metalloproteinases.5-11

Supplier :

Alomone Labs

Target :

MAPK Pathway

Long Description :

U0126 (#U-400) is a highly pure, synthetic, and biologically active compound.

Short Description :

A Specific MAPK Pathway Inhibitor

MW :

380.5

Source :

Synthetic

Molecular formula :

C18H16N6S2.

Effective Concentration :

0.1-100 µM.

Activity :

U0126 specifically inhibits the kinase activity of mitogen activated protein kinase kinase (MAPKK, MEK1 and MEK2). It is less toxic than PD 980591,2.

Reconstitution and Solubility :

DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to two weeks at 4°C or six months at -20°C.Protect from light.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>99%

CAS No :

109511-58-2

Form :

Lyophilized Powder

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

1,4-Diamino-2,3-dicyano-1,4-bis[2- aminophenylthio]butadiene.

more info or order :

Alomone Labs product webpage