UK-78282 hydrochloride | Alomone Labs U-100 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

UK-78282 hydrochloride

catalog :

U-100

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Vivekananda U, Novak P, Bello O, Korchev Y, Krishnakumar S, Volynski K, et al. Kv1.1 channelopathy abolishes presynaptic spike width modulation by subthreshold somatic depolarization. Proc Natl Acad Sci U S A. 2017;114:2395-2400 pubmed publisher

image

image 1 :

Alomone LabsUK-78282 hydrochlorideblocks KV1.3 channels heterologously expressed inXenopusoocytes. - A. Time course of 200 ?MUK-78282 hydrochloride(#U-100) reversible inhibitory action on KV1.3 current amplitude. Ramp stimulation; 100 ms from holding potential -100 mV to +50 mV delivered every 10 seconds.B. Superimposed current traces of control and inhibited current as indicated (taken from the experiment in A).

image 2 :

Alomone Labs 5-(NN-Dimethyl)amiloride hydrochlorideinhibitsallyl isothiocyanate induced calcium signal in TRPA1 expressing cells. - Dose-response curve for the inhibition of human TRPA1 expressing HEK-293 cells loaded with Calcium 5 dye for changes in intracellular Ca2+measurements.Allyl isothiocyanate (100 M) induced calcium signal was inhibited by 50 100 and 200M of 5-(NN-Dimethyl)amiloride hydrochloride(#D-165) with estimated IC50of about 60 M.

product information

CAT :

U-100

SKU :

U-100_10 mg

Product Name :

UK-78282 hydrochloride

Group Type :

Non Antibodies

Product Type :

S. Molecules

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

UK-78282 hydrochloride is a novel piperidine blocker of the T lymphocyte voltage-gated K+ channel, KV1.3 and KV1.4 with IC50 values of 0.28 and 0.17 μM respectively. UK-78282 suppresses T lymphocyte mitogenesis and leads to membrane depolarization. UK-78282 also blocks KV1.4 expressed in heart and brain. UK-78282 preferentially blocks the C-type inactivated state of the KV1.3 channel. KV1.3 mutants containing hydrophobic residues that might prefer to be buried, transition slowly into the C-type inactivated conformation and are insensitive to block by UK-78282, possibly because of the inability of the drug to bind the channel. UK-78282 hydrochloride serves as an experimental tool to probe the conformational changes in KV1.3 leading to C-type inactivation and to further define the critical physiological role of KV1.3 in T lymphocyte activation1.

Supplier :

Alomone Labs

Target :

KV1.3, KV1.4 K+ channels

Long Description :

UK-78282 hydrochloride (#U-100) is a highly pure, synthetic, and biologically active compound.

Short Description :

A Blocker of KV1.3 and KV1.4 K+ Channels

MW :

466.1

Source :

Synthetic

Molecular formula :

C29H36ClNO2.

Effective Concentration :

200 nM - 200 µM.

Activity :

UK-78282 hydrochloride blocks KV1.3 and KV1.4 in T lymphocytes1.

Reconstitution and Solubility :

200 mM in DMSO. Centrifuge all product preparations before use (10000 x g for 1 min).

Solubility :

200 mM in DMSO. Centrifuge all product preparations before use (10000 x g for 1 min).

Storage of solutions :

Up to four weeks at 4°C or three months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>95%

CAS No :

136647-02-4

Form :

Lyophilized Powder

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

4-(benzhydryloxymethyl)-1-[3-(4-methoxyphenyl)propyl]piperidine;hydrochloride.

more info or order :

Alomone Labs product webpage