Tyrphostin AG 1296 | Alomone Labs T-770 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Tyrphostin AG 1296

catalog :

T-770

more info or order :

Alomone Labs product webpage

image

image 1 :

image 2 :

Alomone Labs Tyrphostin AG 1296 inhibits the kinase activity of PDGF receptor in 3T3-L1 cells. - Cells were serum starved for 3 h. The cells were then incubated for 30 min with various concentrations ofTyrphostin AG 1296(#T-770) and stimulated with 10 ng/ml PDGF-BB. Cell proteins were resolved by SDS-PAGE and probed with anti-phospho-PDGFR (upper panel) and with antiphospho ERK1/2 (lower panel). Inhibition of ERK1/2 phosphorylation was dose-dependent.

product information

CAT :

T-770

SKU :

T-770_1 mg

Product Name :

Tyrphostin AG 1296

Group Type :

Non Antibodies

Product Type :

S. Molecules

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

Tyrphostin AG1296 is a tyrosine kinase blocker which selectively inhibits platelet-derived growth factor (PDGF) a- and b-receptor kinases and the PDGF-dependent signaling in many cell types. Tyrphostin AG1296 also inhibits the PDGF dependent transactivation of the P2Y and Endothelin receptors-evoked cell proliferation and MAPK activation.1-4This PDGF receptor (PDGFR) blocker has no effect on epidermal growth factor (EGF) receptor autophosphorylation; weak effects on DNA synthesis stimulated by insulin, by EGF, or by a combination of both; and over an order of magnitude weaker blocking effect on fibroblast growth factor-dependent (FGF) DNA synthesis.5-8 Tyrphostin AG1296 also potently inhibits the signaling of stem cell factor (SCF) receptor (c-Kit) and its upstream effector Kit receptor tyrosine kinase and the tyrosine kinase activity of FLT3-ITD which both have a strong sequence similarity with other members of the class III receptor tyrosine kinase but has no effect on the autophosphorylation of vascular endothelial growth factor (VEGF) receptor. 7,8

Supplier :

Alomone Labs

Target :

PDGF Receptor Tyrosine Kinase

Long Description :

Tyrphostin AG 1296 (#T-770) is a highly pure, synthetic, and biologically active compound.

Short Description :

A PDGF Receptor Tyrosine Kinase Inhibitor

MW :

266.3

Source :

Synthetic

Molecular formula :

C16H14N2O2.

Effective Concentration :

>1-5 µM.

Activity :

Tyrphostin AG 1296 is a highly selective blocker of platelet-derived growth factor (PDGF) α and β receptor tyrosine kinase1-3. It could also inhibit the signalling of stem cell factor (SCF) receptor (c-kit) and FLT3-ITD receptor4,5.

Reconstitution and Solubility :

DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

DMSO. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to two weeks at 4°C or six months at -20°C. Protect from light.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>98%

CAS No :

146535-11-7

Form :

Lyophilized Powder

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

6,7-Dimethyl-2-phenylquinoxaline.

more info or order :

Alomone Labs product webpage