Tetrodotoxin citrate free | Alomone Labs T-500 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Tetrodotoxin citrate free

catalog :

T-500

more info or order :

Alomone Labs product webpage

citations: 23

Reference
Xu Y, Lu Y, Cassidy R, Mangieri L, Zhu C, Huang X, et al. Identification of a neurocircuit underlying regulation of feeding by stress-related emotional responses. Nat Commun. 2019;10:3446 pubmed publisher
Yang C, Yu T, Wen W, Ling P, Hsu K. Conditional Deletion of CC2D1A Reduces Hippocampal Synaptic Plasticity and Impairs Cognitive Function through Rac1 Hyperactivation. J Neurosci. 2019;39:4959-4975 pubmed publisher
Bories C, Arsenault D, Lemire M, Tremblay C, De Koninck Y, Calon F. Transgenic autoinhibition of p21-activated kinase exacerbates synaptic impairments and fronto-dependent behavioral deficits in an animal model of Alzheimer's disease. Aging (Albany NY). 2017;9:1386-1403 pubmed publisher
Hellmer C, Zhou Y, Fyk Kolodziej B, Hu Z, Ichinose T. Morphological and physiological analysis of type-5 and other bipolar cells in the Mouse Retina. Neuroscience. 2016;315:246-58 pubmed publisher
Wen H, Linhoff M, Hubbard J, Nelson N, Stensland D, Dallman J, et al. Zebrafish calls for reinterpretation for the roles of P/Q calcium channels in neuromuscular transmission. J Neurosci. 2013;33:7384-92 pubmed publisher
Bant J, Aman T, Raman I. Antagonism of lidocaine inhibition by open-channel blockers that generate resurgent Na current. J Neurosci. 2013;33:4976-87 pubmed publisher
Browning K, Fortna S, Hajnal A. Roux-en-Y gastric bypass reverses the effects of diet-induced obesity to inhibit the responsiveness of central vagal motoneurones. J Physiol. 2013;591:2357-72 pubmed publisher
Ran I, Gkogkas C, Vasuta C, Tartas M, Khoutorsky A, Laplante I, et al. Selective regulation of GluA subunit synthesis and AMPA receptor-mediated synaptic function and plasticity by the translation repressor 4E-BP2 in hippocampal pyramidal cells. J Neurosci. 2013;33:1872-86 pubmed publisher
Browning K, Babic T, Holmes G, Swartz E, Travagli R. A critical re-evaluation of the specificity of action of perivagal capsaicin. J Physiol. 2013;591:1563-80 pubmed publisher
Dvorzhak A, Semtner M, Faber D, Grantyn R. Tonic mGluR5/CB1-dependent suppression of inhibition as a pathophysiological hallmark in the striatum of mice carrying a mutant form of huntingtin. J Physiol. 2013;591:1145-66 pubmed publisher
PEREZ G, Desai M, Anderson S, Scornik F. Large-conductance calcium-activated potassium current modulates excitability in isolated canine intracardiac neurons. Am J Physiol Cell Physiol. 2013;304:C280-6 pubmed publisher
Goutman J. Transmitter release from cochlear hair cells is phase locked to cyclic stimuli of different intensities and frequencies. J Neurosci. 2012;32:17025-35a pubmed publisher
Gkogkas C, Khoutorsky A, Ran I, Rampakakis E, Nevarko T, Weatherill D, et al. Autism-related deficits via dysregulated eIF4E-dependent translational control. Nature. 2013;493:371-7 pubmed publisher
Colgan L, Cavolo S, Commons K, Levitan E. Action potential-independent and pharmacologically unique vesicular serotonin release from dendrites. J Neurosci. 2012;32:15737-46 pubmed publisher
Lu V, Puhl H, Ikeda S. N-Arachidonyl glycine does not activate G protein-coupled receptor 18 signaling via canonical pathways. Mol Pharmacol. 2013;83:267-82 pubmed publisher
Luo R, Janssen M, Partridge J, Vicini S. Direct and GABA-mediated indirect effects of nicotinic ACh receptor agonists on striatal neurones. J Physiol. 2013;591:203-17 pubmed publisher
Askar S, Bingen B, Schalij M, Swildens J, Atsma D, Schutte C, et al. Similar arrhythmicity in hypertrophic and fibrotic cardiac cultures caused by distinct substrate-specific mechanisms. Cardiovasc Res. 2013;97:171-81 pubmed publisher
Kohlmeier K, Ishibashi M, Wess J, Bickford M, Leonard C. Knockouts reveal overlapping functions of M(2) and M(4) muscarinic receptors and evidence for a local glutamatergic circuit within the laterodorsal tegmental nucleus. J Neurophysiol. 2012;108:2751-66 pubmed publisher
Janiek R, Polakova E, Pavelkova J, Zahradnik I, Zahradnikova A. Calcium spike variability in cardiac myocytes results from activation of small cohorts of ryanodine receptor 2 channels. J Physiol. 2012;590:5091-106 pubmed publisher
Song W, Xiao Y, Chen H, Ashpole N, Piekarz A, Ma P, et al. The human Nav1.5 F1486 deletion associated with long QT syndrome leads to impaired sodium channel inactivation and reduced lidocaine sensitivity. J Physiol. 2012;590:5123-39 pubmed publisher
Akemann W, Mutoh H, Perron A, Park Y, Iwamoto Y, Knopfel T. Imaging neural circuit dynamics with a voltage-sensitive fluorescent protein. J Neurophysiol. 2012;108:2323-37 pubmed publisher
Guzman J, Sanchez Padilla J, Chan C, Surmeier D. Robust pacemaking in substantia nigra dopaminergic neurons. J Neurosci. 2009;29:11011-9 pubmed publisher
Wan S, Browning K, Coleman F, Sutton G, Zheng H, Butler A, et al. Presynaptic melanocortin-4 receptors on vagal afferent fibers modulate the excitability of rat nucleus tractus solitarius neurons. J Neurosci. 2008;28:4957-66 pubmed publisher

image

image 1 :

Alomone Labs Tetrodotoxincitrate free inhibits native NaVcurrents in PC12 and ND7-23cells. - Tetrodotoxincitrate free(#T-500) inhibits PC12 (top) and ND7-23 (bottom) cells at a concentration of 60 nM. Left graphs show the time course of inward current amplitude upon bath application (marked by the horizontal bars) and wash of TTX. Membrane potential was clamped to -120 mV and currents were elicited by 20 ms test pulses to -10 or -20 mV (upper and lower graphs respectively) delivered every 10 sec. Right graphs show the superimposed traces of control current (red) and during perfusion of TTX (black).

image 2 :

Alomone LabsChembridge-5861528inhibitsAllyl isothiocyanate induced calcium signal in TRPA1 expressing cells. - Dose-response curve for the inhibition of human TRPA1 expressing HEK-293 cells loaded with Calcium 5 dye for changes in intracellular Ca2+ measurements. Allyl isothiocyanate (100 M)-induced calcium signal was inhibited by 5 M Chembridge-5861528 (#C-235) with estimated IC50 of about 4 M.

product information

CAT :

T-500

SKU :

T-500_1 mg

Product Name :

Tetrodotoxin citrate free

Group Type :

Non Antibodies

Product Type :

S. Molecules

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

Tetrodotoxin (TTX) is an established potent and selective blocker of some NaV channels.1 Moreover, TTX sensitivity is used to define subclasses of NaV channels.2 It is widely used both to probe NaV activity and to reduce or block excitability in neurophysiological preparations.

Supplier :

Alomone Labs

Target :

Various NaV Na+ channels

Long Description :

Tetrodotoxin citrate free (#T-500) is a highly pure, natural, and biologically active compound.

Short Description :

A Classic Blocker of a Subset of NaV Channels

MW :

319.28

Synonyms :

TTX

Source :

Natural

Molecular formula :

C11H17N3O8.

Effective Concentration :

100 nM - 1 µM.

Activity :

Tetrodotoxin is a potent and selective blocker of a subclass of NaV channels1, and is often used to define subclasses of NaV channels2.

Reconstitution and Solubility :

Acidic buffer (pH 4.8). Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

Acidic buffer (pH 4.8). Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to one week at 4°C or six months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>99% (HPLC)

CAS No :

4368-28-9

Form :

Lyophilized Powder

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

Octahydro-12-(hydroxymethyl)-2-imino-5,9:7,10adimethano-10aH-[1,3]dioxocino[6,5-d]pyrimidine-4,7,10,11,12-pentol.

more info or order :

Alomone Labs product webpage