Taipoxin | Alomone Labs T-460 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Taipoxin

catalog :

T-460

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs Taipoxin changes the actin filaments distributionin the growing neurite tip bulge of differentiated PC12 cells. - Cells were differentiated with 50 ng/mlNative mouse NGF 2.5S protein (99%)(#N-240) for 3 days and stimulated with control vehicle (A)or10 nM (B)and 20 nM (C)Taipoxin(#T-460)for 1h. The figure presents actin filamentsstained with Rhodamine conjugated phalloidin.

image 2 :

Alomone Labs Taipoxin changes the outer membrane distribution of Syntaxin-1 and Synaptotagmin-1 in dorsal root ganglia (DRG) neurons. - Rat DRG neuron cell cultures were treated with or without 20 nMTaipoxin(#T-460) for one hour. The cells were labeled withAnti-Syntaxin 1 Antibody(#ANR-002) andAnti-Synaptotagmin-1 (SYT1) Antibody(#ANR-003) followed by goat anti- rabbit-AlexaFluor-555 secondary antibody.

product information

cat :

T-460

SKU :

T-460_0.5 mg

Product Name :

Taipoxin

Group Type :

Non Antibodies

Product Type :

Proteins

Applications :

Immunocytochemistry, Neurite outgrowth assay

Formulation :

Lyophilized from filtrated Ammonium acetate solution. May contain acetate as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Oxyuranus scutellatus scutellatus (Australian taipan) (Coastal taipan)

Source :

Natural protein

Gene ID :

NPTX1,NPTX2,NPTXR

Product Page - Scientific background :

Taipoxin is a lethal neurotoxin protein isolated from the venom of the Australian taipan Oxyuranus s. scutellatus (LD50= 2 µg/kg in mouse). Taipoxin is a non-covalent ternary glycoprotein which is completely dissociated at low pH or high ionic strength. One of the subunits has Phospholipase A2 activity1,2.Intoxicated animals die of asphyxia caused by a complete inhibition of neurotransmitter release and neuromuscular junction blockage of the respiratory muscles. In the central nervous system taipoxin blocks synaptic vesicle recycling by inhibiting the neuronal uptake pathway through the interaction with neuronal Pentraxin system components during synapse formation and remodeling1,3-5.In neuronal cultures, Taipoxin facilitates Ca2+-dependent synaptic vesicle exocytosis and causes a complete depletion of stored neurotransmitter, resulting in synaptic transmission blockage. Taipoxin, at nanomolar range, causes swelling of nerve terminus and redistribution of synaptic vesicle proteins7.

Supplier :

Alomone Labs

Long Description :

A Blocker of Synaptic Vesicle Recycling

Short Description :

A Blocker of Synaptic Vesicle Recycling

MW :

46 kDa

Synonyms :

Taipan toxin

Modifications :

Highly disulfide bridged protein, glycosylated

Effective Concentration :

10 - 50 nM

Activity :

Taipoxin gradually and completely stops induced and spontaneous release of acetylcholine from nerve terminals1. Taipoxin is considered to be the most potent toxin.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥97% (HPLC)

CAS No :

52019-39-3

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

alpha, beta and gamma chains non-covalently linkedS

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage