Trichostatin A | Alomone Labs T-300 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Trichostatin A

catalog :

T-300

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Nagao Y, Sakamoto M, Chinen T, Okada Y, Takao D. Robust classification of cell cycle phase and biological feature extraction by image-based deep learning. Mol Biol Cell. 2020;31:1346-1354 pubmed publisher
Lyu C, Mulder J, Barde S, Sahlholm K, Zeberg H, Nilsson J, et al. G protein-gated inwardly rectifying potassium channel subunits 1 and 2 are down-regulated in rat dorsal root ganglion neurons and spinal cord after peripheral axotomy. Mol Pain. 2015;11:44 pubmed publisher

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Alomone Labs Trichostatin A inhibits histone deacetylases in NIH/3T3 cells. - Cells from logarithmic were incubated overnight with different concentrations of Trichostatin A (#T-300). Cell proteins were resolved by SDS-PAGE and probed with anti-acetylated histone antibodies.

product information

CAT :

T-300

SKU :

T-300_0.5 mg

Product Name :

Trichostatin A

Group Type :

Non Antibodies

Product Type :

S. Molecules

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Product Page - Scientific background :

Trichostatin A (TSA), an antifungal antibiotic derived from Streptomyces, is a potent and reversible inhibitor of histone deacetylase.1 TSA causes accumulation of highly acetylated histone molecules in mammalian cells,2 that leads to chromatin relaxation and modulation of gene transcription/expression.3TSA blocked cell cycle progression at the G1 phase in HeLa cells.4 In NIH 3T3 cells, it induced reversion of oncogenic ras-transformed cells to a normal morphology.5 TSA did not induce apoptosis in primary rat hepatocytes,6 however, it induced cell-cycle arrest and apoptosis in human hepatoma cell lines (HepG2, Huh-7).7Unlike sodium butyrate, Trichostatin A action is very specific and reversible and thus, became a widely used tool to study the consequences of histone acetylation in vitro and in vivo.TSA strongly inhibited the cellular growth of nine pancreatic adenocarcinoma cell lines. It induced up-regulation of p21 (WAF1/CIP1), cell cycle arrest at G2, and apoptosis. Therefore, TSA may have a potential utility in treatment of pancreatic cancer.8Recently, TSA was reported as a potent inhibitor of Gelatinase A, a matrix metalloproteinase implicated in metastasis,9 and it also inhibited the secretion of vascular endothelial growth factor (VEGF) from human glioblastoma cells.10 Preclinical evaluation of TSA in combination with DNA methylation inhibitors, demonstrated a synergistic anti-neoplastic effect against myeloid leukemic cells.11

Supplier :

Alomone Labs

Long Description :

Trichostatin A (#T-300) is a highly pure, natural, and biologically active compound.

Short Description :

A Powerful Histone Deacetylase Inhibitor

MW :

302.4

Synonyms :

Antibiotic A300, TSA

Source :

Natural

Molecular formula :

C17H22N2O3.

Effective Concentration :

100 nM - 1 µM.

Activity :

Trichostatin A, an antifungal antibiotic, irreversibly inhibits histone deacetylase1. It could also promote apoptosis2 and in some cases even inhibits the proliferation of some cancer cells3.

Reconstitution and Solubility :

DMSO or ethanol. Centrifuge all product preparations before use (10000 x g 5 min).

Solubility :

DMSO or ethanol. Centrifuge all product preparations before use (10000 x g 5 min).

Storage of solutions :

Up to two weeks at 4°C or six months at -20°C.

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.

Purity :

>98%

CAS No :

58880-19-6

Form :

Lyophilized powder.

Comment :

Contact Alomone Labs for technical support and product customization

UNSPSC :

41116134

Bioassay Tested :

Yes

Chemical Name :

(2E,4E,6R)-7-(4-(Dimethylamino)phenyl)-N-hydroxy-4,6- di methyl-7-oxo-2,4-heptadienamide.

more info or order :

Alomone Labs product webpage