product summary
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company name :
Alomone Labs
product type :
chemical
product name :
TTA-P2
catalog :
T-155
more info or order :
Alomone Labs product webpage
citations: 24
Reference
Feseha S, Timić Stamenić T, Wallace D, Tamag C, Yang L, Pan J, et al. Global genetic deletion of CaV3.3 channels facilitates anaesthetic induction and enhances isoflurane-sparing effects of T-type calcium channel blockers. Sci Rep. 2020;10:21510 pubmed publisher
Turecek J, Regehr W. Cerebellar and vestibular nuclear synapses in the inferior olive have distinct release kinetics and neurotransmitters. elife. 2020;9: pubmed publisher
Hanemaaijer N, Popovic M, Wilders X, Grasman S, Pavón Arocas O, Kole M. Ca2+ entry through NaV channels generates submillisecond axonal Ca2+ signaling. elife. 2020;9: pubmed publisher
Woodward T, Tesic V, Stamenić T, Jevtovic Todorovic V, Todorovic S. Pharmacological Antagonism of T-Type Calcium Channels Constrains Rebound Burst Firing in Two Distinct Subpopulations of GABA Neurons in the Rat Ventral Tegmental Area: Implications for α-Lipoic Acid. Front Pharmacol. 2019;10:1402 pubmed publisher
Yang T, He M, Zhang H, Barrett P, Hu C. L- and T-type calcium channels control aldosterone production from human adrenals. J Endocrinol. 2020;244:237-247 pubmed publisher
McNally B, Plante A, Meredith A. Diurnal properties of voltage-gated Ca2+ currents in suprachiasmatic nucleus and roles in action potential firing. J Physiol. 2020;598:1775-1790 pubmed publisher
Nigam A, Hargus N, Barker B, Ottolini M, Hounshell J, Bertram E, et al. Inhibition of T-Type calcium channels in mEC layer II stellate neurons reduces neuronal hyperexcitability associated with epilepsy. Epilepsy Res. 2019;154:132-138 pubmed publisher
Aiba I, Noebels J. Adrenergic agonist induces rhythmic firing in quiescent cardiac preganglionic neurons in nucleus ambiguous via activation of intrinsic membrane excitability. J Neurophysiol. 2019;121:1266-1278 pubmed publisher
Wu J, Peng S, Xiao L, Cheng X, Kuang H, Zhu M, et al. Cell-Type Specific Distribution of T-Type Calcium Currents in Lamina II Neurons of the Rat Spinal Cord. Front Cell Neurosci. 2018;12:370 pubmed publisher
Moutal A, Sun L, Yang X, Li W, Cai S, Luo S, et al. CRMP2-Neurofibromin Interface Drives NF1-related Pain. Neuroscience. 2018;381:79-90 pubmed publisher
Tracy M, Tesic V, Stamenić T, Joksimovic S, Busquet N, Jevtovic Todorovic V, et al. CaV3.1 isoform of T-type calcium channels supports excitability of rat and mouse ventral tegmental area neurons. Neuropharmacology. 2018;135:343-354 pubmed publisher
Chen W, Chi Y, Kang X, Liu Q, Zhang H, Li Z, et al. Accumulation of Cav3.2 T-type Calcium Channels in the Uninjured Sural Nerve Contributes to Neuropathic Pain in Rats with Spared Nerve Injury. Front Mol Neurosci. 2018;11:24 pubmed publisher
Irie T, Trussell L. Double-Nanodomain Coupling of Calcium Channels, Ryanodine Receptors, and BK Channels Controls the Generation of Burst Firing. Neuron. 2017;96:856-870.e4 pubmed publisher
Zhao Y, Sivaji S, Chiang M, Ali H, Zukowski M, Ali S, et al. Amyloid Beta Peptides Block New Synapse Assembly by Nogo Receptor-Mediated Inhibition of T-Type Calcium Channels. Neuron. 2017;96:355-372.e6 pubmed publisher
Yang C, McKenna J, Brown R. Intrinsic membrane properties and cholinergic modulation of mouse basal forebrain glutamatergic neurons in vitro. Neuroscience. 2017;352:249-261 pubmed publisher
Huang C, Lien C, Cheng C, Yen T, Chen C, Tsaur M. K+ Channel Kv3.4 Is Essential for Axon Growth by Limiting the Influx of Ca2+ into Growth Cones. J Neurosci. 2017;37:4433-4449 pubmed publisher
Kisiswa L, Erice C, Ferron L, Wyatt S, Osório C, Dolphin A, et al. T-type Ca2+ channels are required for enhanced sympathetic axon growth by TNFα reverse signalling. Open Biol. 2017;7: pubmed publisher
Xie J, Chew L, Yang X, Wang Y, Qu C, Wang Y, et al. Sustained relief of ongoing experimental neuropathic pain by a CRMP2 peptide aptamer with low abuse potential. Pain. 2016;157:2124-40 pubmed publisher
Yang T, Zhang H, Liang Q, Shi Y, Mei Y, Barrett P, et al. Small-Conductance Ca2+-Activated Potassium Channels Negatively Regulate Aldosterone Secretion in Human Adrenocortical Cells. Hypertension. 2016;68:785-95 pubmed publisher
Barzan R, Pfeiffer F, Kukley M. N- and L-Type Voltage-Gated Calcium Channels Mediate Fast Calcium Transients in Axonal Shafts of Mouse Peripheral Nerve. Front Cell Neurosci. 2016;10:135 pubmed publisher
Kortus S, Srinivasan C, Forostyak O, Zapotocky M, Ueta Y, Sykova E, et al. Sodium-calcium exchanger and R-type Ca(2+) channels mediate spontaneous [Ca(2+)]i oscillations in magnocellular neurones of the rat supraoptic nucleus. Cell Calcium. 2016;59:289-98 pubmed publisher
Seol M, Kuner T. Ionotropic glutamate receptor GluA4 and T-type calcium channel Cav 3.1 subunits control key aspects of synaptic transmission at the mouse L5B-POm giant synapse. Eur J Neurosci. 2015;42:3033-44 pubmed publisher
Scott A, Zhang M, Nurse C. Enhanced BDNF signalling following chronic hypoxia potentiates catecholamine release from cultured rat adrenal chromaffin cells. J Physiol. 2015;593:3281-99 pubmed publisher
Enyeart J, Enyeart J. Adrenal fasciculata cells express T-type and rapidly and slowly activating L-type Ca2+ channels that regulate cortisol secretion. Am J Physiol Cell Physiol. 2015;308:C899-918 pubmed publisher
image
image 1 :
Alomone Labs T-155 image 1
Alomone Labs TTA-P2 inhibits T-type CaVchannels heterologously expressed inXenopusoocytes. - A. Time course of CaV3.2 peak current amplitude elicited by 100 ms voltage step from holding potential of -100 mV to -30 mV delivered every 10 seconds. Application of 5 MTTA-P2(#T-155) inhibits the CaV3.2 current in a reversible manner (indicated by the horizontal bar). B. Representative current traces before and during application of 5 M TTA-P2 as indicated.
image 2 :
Alomone Labs T-155 image 2
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: - 12.Anti-Nicotinic Acetylcholine Receptor ?9 (CHRNA9) Antibody(#ANC-019) (1:400).34. Anti-Nicotinic Acetylcholine Receptor ?9 (CHRNA9) Antibody preincubated with the negative control antigen.
product information
CAT :
T-155
SKU :
T-155_10 mg
Product Name :
TTA-P2
Group Type :
Non Antibodies
Product Type :
S. Molecules
Storage After Reconstitution :
The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
Shipping and storage :
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Product Page - Scientific background :
TTA-P2 is a novel, potent and selective T-type CaV channel blocker. In thalamocortical and reticular neurons, it blocks T-type currents with an IC50 of 22 nM without affecting high voltage Ca2+ channels1. In rat DRGs, it blocks T-type currents with an IC50 of 100 nM and stabilizes the channel in the inactive state2.TTA-P2 has the potential of acting as an antinociceptive agent2.
Supplier :
Alomone Labs
Target :
T-type CaV channels
Long Description :
TTA-P2 (#T-155) is a highly pure, synthetic, and biologically active compound.
Short Description :
A Blocker of T-Type CaV Channels
MW :
431
Source :
Synthetic
Molecular formula :
C21H29Cl2FN2O2.
Effective Concentration :
0.05-10 µM.
Activity :
TTA-P2 blocks T-type currents in DRGs with IC50 of 100 nM and 22 nM in thalamocortical and reticular neurons1,2.
Reconstitution and Solubility :
Up to 25mM in DMSO. Centrifuge all product preparations before use (10000 x g for 1 min).
Solubility :
Up to 25mM in DMSO. Centrifuge all product preparations before use (10000 x g for 1 min).
Storage of solutions :
Up to four weeks at 4°C or three months at -20°C.
Lead Time :
1-2 Business Days
Country of origin :
Israel/IL
Applications key :
Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.
Purity :
>96% (HPLC)
CAS No :
918430-49-6
Form :
Lyophilized powder.
Comment :
Contact Alomone Labs for technical support and product customization
UNSPSC :
41116134
Bioassay Tested :
Yes
Chemical Name :
3,5-dichloro-N-[1-(2,2-dimethyl-tetrahydro-pyran-4-ylmethyl)-4-fluoro-piperidin-4-ylmethyl]-benzamide. Racemic mixture.
more info or order :
Alomone Labs product webpage
company information
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com
972 2 531 8002
headquarters: Israel