chemical

Tetanus Toxin

T-150

T-150_10 mcg

Tetanus Toxin

Non Antibodies

Proteins

P04958

https://www.uniprot.org/uniprotkb/P04958/entry

Lyophilized Powder.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. Soluble in any conventional buffer containing 0.01 KH2PO4, 0.1% gelatin and/or 0.1% bovine serum albumin (BSA). The use of gelatin or 0.01% BSA coated/treated glassware is recommended. Centrifuge all product preparations before use (10000 x g 5 min).

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes). The lyophilizate may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. Soluble in any conventional buffer containing 0.01 KH2PO4, 0.1% gelatin and/or 0.1% bovine serum albumin (BSA). The use of gelatin or 0.01% BSA coated/treated glassware is recommended. Centrifuge all product preparations before use (10000 x g 5 min).

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Clostridium tetani

Natural protein

VAMP2

Tetanus toxin is a 150 kD protein produced by the gram positive bacteria Clostridium tetani under anaerobic condition1. Tetanus toxin is a hetrodimer of A-chain (50 kD) and B-chain (100 kD) linked by one disulfide bond2,3. The A-chain has a zinc endopeptidase activity while the B-chain has a role in toxin anchoring to neuronal membranes4.Tetanus toxin spreads in the body through the lymphatic and vascular systems to the neuromuscular junction, internalized to peripheral neuron synapses, and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons to the CNS5. There, it inhibits neurotransmitter release by the specific degradation of the vesicle-secretory protein-Synaptobrevin6.

Alomone Labs

An Inhibitor of Neurotransmitter Release

An Inhibitor of Neurotransmitter Release

150 kDa

Tentoxylysin

Disulfide bonds between Cys439-Cys467 and Cys1077-Cys1093

1 - 100 nM

Tetanus Toxin inhibits neurotransmitter release by cleaving synaptobrevin-2 in a zinc-dependent manner1,2.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

1-2 Business Days

Israel/IL

≥97% (HPLC)

676570-37-9

Lyophilized

Contact Alomone Labs for technical support and product customization

Yes

12352202

no

Electrophysiology