Waglerin-1 | Alomone Labs STW-001 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Waglerin-1

catalog :

STW-001

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone LabsWaglerin-I inhibits muscle embryonic ?1/?1/?/?nAChR currentsheterologously expressed inXenopusoocytes. - A. Time course ofWaglerin-1(#STW-001) action on muscle ?1/?1/?/? nAChR. Current amplitudes were plotted as a function of time. Membrane potential was held at -80 mV and oocytes were stimulated by exposure to 10 ?M acetylcholine every 100 seconds. 10 M (for 3 min) Waglerin-1 were perfused during the period marked by the bar as indicated. B. Superimposed traces of muscle nAChR channel current in the absence (black) and presence (green) of 10 M Waglerin-1 (taken from the experiment in A).

product information

cat :

STW-001

SKU :

STW-001_1 mg

Product Name :

Waglerin-1

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P24335

Accession Number :

https://www.uniprot.org/uniprotkb/P24335/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Tropidolaemus wagleri (Wagler's pit viper) (Trimeresurus wagleri)

Source :

Synthetic peptide

Gene ID :

CHRNA1,CHRNB1,CHRND,CHRNE,GABR

Product Page - Scientific background :

Waglerin-1 is a 22 amino acid peptide toxin isolated from the Southeast Asian snake Trimeresurus wagleri1. Two other similar peptides named Waglerin-2 and 3 are isolated from the same snake species1.Waglerin-1 selectively blocks the nAChRε subunit with an IC50 of 50 nM2 and is a potent blocker of muscle nAChR3. Waglerin-1 is also a modulator of GABA(A) receptors with potentiating and suppressing effects. Its IC50 and EC50 value is 24 μM4.

Supplier :

Alomone Labs

Target :

nAChR, GABA(A) receptors

Long Description :

A Potent Antagonist of nAChR and a Modulator of GABA(A) Receptors

Short Description :

A Potent Antagonist of nAChR and a Modulator of GABA(A) Receptors

MW :

2520 Da

Synonyms :

Waglerin-I, Wtx-1, Lethal peptide I

Modifications :

Disulfide bond between: Cys9-Cys13

Molecular formula :

C112H175N37O26S2

Effective Concentration :

1- 5 µM

Activity :

Waglerin-1 blocks ε subunit of muscle adult nAChR and modulates rodent GABA(A)/GABRA1 receptors1,2.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥99% (HPLC)

CAS No :

145038-84-2

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

GGKPDLRPCHPPCHYIPRPKPR-OH

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage