Tertiapin-LQ | Alomone Labs STT-220 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Tertiapin-LQ

catalog :

STT-220

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone LabsTertiapin-LQ inhibits Kir1.1 channels heterologously expressed inXenopusoocytes. - A continuous current trace of Kir1.1 channels was recorded at a holding potential of -80 mV. Channel activation was achieved by a continuous application of high K+containing solution (indicated by the bottom bar). Kir1.1 currents are inhibited by increasing concentrations ofTertiapin-LQ(#STT-220) applied for 2 min each (100 nM 200 nM 500 nM and 1M as indicated by the top green bars).

product information

cat :

STT-220

SKU :

STT-220_0.5 mg

Product Name :

Tertiapin-LQ

Group Type :

Non Antibodies

Product Type :

Proteins

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Apis mellifera (Honeybee)

Source :

Synthetic peptide

Gene ID :

KCNJ1,KCNJ3,KCNJ5

Product Page - Scientific background :

Inward-rectifier K+ (Kir) channels play many important biological roles and are emerging as important therapeutic targets. Tertiapin (TPN), a toxin present in honey bee venom (Apis mellifera) inhibits certain subtypes of inward-rectifier K+ channels1. TPN is a highly compact peptide of 21 residues. Its C-terminal portion (histidine 12 to glycine 19) adopts an α-helical structure, whereas the N-terminal half acquires extended conformations. Two pairs of disulfide bonds help to hold the two parts together2. Tertiapin inhibits ROMK1 (Kir1.1) and G-protein-gated channel (GIRK1/4) (Kir3.1/3.4) channels with IC50 values of 8.6 and 2.0 nM respectively and is selective over Kir2.1 channels3. In accordance, it was shown to inhibit acetylcholine induced K+ currents in mammalian cardiomyocytes4.Tertiapin-LQ is a tertiapin-Q derivative that blocks Kir1.1 channels. It does so by binding to the external vestibule of the K+-conduction pore that is formed by the linker between the first and second transmembrane (M1-M2) segments1.

Supplier :

Alomone Labs

Target :

Kir1.1, Kir3.1, Kir3.4 K+ channels

Long Description :

A Blocker of Kir1.1, Kir3.1 and Kir3.4 K+ Channels

Short Description :

A Blocker of Kir1.1, Kir3.1 and Kir3.4 K+ Channels

MW :

2428 Da

Synonyms :

Tertiapin LQ

Modifications :

Disulfide bonds between: Cys3-Cys14 and Cys5-Cys18 Lys21 -C-terminal amidation

Molecular formula :

C106H179N33O24S4

Effective Concentration :

100 nM

Activity :

Tertiapin-LQ is a selective blocker of Kir1.1 channels.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

ALCNCNRIIIPLQCWKKCGKK-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage