chemical

Tertiapin-RQ

STT-180

STT-180_0.1 mg

Tertiapin-RQ

Non Antibodies

Proteins

Electrophysiology

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Apis mellifera (Honeybee)

Synthetic peptide

KCNJ1,KCNJ3,KCNJ5

Tertiapin-RQ (TPN-RQ) is a derivative of Tertiapin (#STT-250), a native toxin originally isolated from the venom of the bee, Apis mellifera. Tertiapin blocks a range of inward-rectifier K+ channels (Kir), in particular ROMK1 (Kir1.1) and GIRK (Kir3 family)1. Tertiapin also inhibits acetylcholine induced K+ currents in mammalian cardiomyocytes2,3. Compared to Tertiapin, TPN-RQ contains an Arg substitution at Ile-8 and the oxidation-liable Met-13 is substituted with a Gln residue. Similar to Tertiapin, TPN-RQ blocks ROMK1 and GIRK channels4.Kir channels have diverse physiological functions based on their type and their location. ROMK1 is expressed in the kidneys and brain, but its role in the central nervous system remains unknown. Recent studies suggested that the ROMK1 channel plays a role in mental illness. Consistent with this hypothesis, TPN-RQ suppressed neuronal activity and exhibited an antidepressive and anxiogenic effects in animal models4.

Alomone Labs

Kir1.1, Kir3.1, Kir3.4 K+ channels

A Blocker of Kir1.1, Kir3.1 and Kir3.4 K+ Channels

A Blocker of Kir1.1, Kir3.1 and Kir3.4 K+ Channels

2496 Da

TPN-RQ

Disulfide bonds between: Cys3-Cys14 and Cys5-Cys18

C106H175N37O25S4

200 nM

Tertiapin-RQ blocks a range of inward rectifier K+ channels (Kir), in particular ROMK1 and GIRK, with no significant effect on Kir2 family. Additionally, TPN-RQ was shown to have an antidepressive and anxiogenic effect in animal model1.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

1-2 Business Days

Israel/IL

≥98% (HPLC)

Lyophilized

Contact Alomone Labs for technical support and product customization

ALCNCNRRIIPHQCWKKCGKK-OH

Yes

12352202

yes

no