Tertiapin-Q-ATTO Fluor-633 | Alomone Labs STT-170-FR product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Tertiapin-Q-ATTO Fluor-633

catalog :

STT-170-FR

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone LabsTertiapin-Q-ATTO-633binds GIRK1/GIRK4-GFP transfected HEK293T cells. - Living cells were incubated in the presence of 100 nMTertiapin-Q-ATTO-633(#STT-170-FR). A. Visible light of cells. B. GIRK1/GIRK4-GFP transfected cells (green). C. Tertiapin-Q-ATTO-633 (red). The pictures are a kind gift from the lab of Prof. Eithan Reuveny Weizmann Institute Israel.

image 2 :

Alomone LabsTertiapin-Q-ATTO-633 inhibits Kir3.2 channel heterologously expressed inXenopusoocytes. - A continuous current trace recorded at a holding potential of -80 mV. Kir3.2 currents are downward reflections activated by high K+containing solution. While activated 50 nM and 100 nM ofTertiapin-Q-ATTO-633(#STT-170-FR) were applied for 2 min (indicated as bars).

image 3 :

product information

cat :

STT-170-FR

SKU :

STT-170-FR_5 mcg

Product Name :

Tertiapin-Q-ATTO Fluor-633

Group Type :

Non Antibodies

Product Type :

Proteins

Applications :

Electrophysiology, Live cell imaging, Immunofluorescence, Fluorescence staining, Direct flow cytometry

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is lyophilized in 0.5 ml conical vial. The product is soluble in pure water to high-micromolar concentrations (5 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity. Avoid exposure to light.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is lyophilized in 0.5 ml conical vial. The product is soluble in pure water to high-micromolar concentrations (5 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity. Avoid exposure to light.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Apis mellifera (Honeybee)

Source :

Modified synthetic peptide

Gene ID :

KCNJ1,KCNJ3,KCNJ5, KCNJ6, KCNMA1

Product Page - Scientific background :

Tertiapin, the native toxin, was originally isolated from European honey bee Apis mellifera venoM Native and synthetic Tertiapin blocks a range of inward rectifier K+ channels (Kir), in particular ROMK1 (Kir1.1, IC50 = 2 nM) and GIRK (Kir3 family, IC50 for the Kir3.1/3.4 heteromer was 8.6 nM) but with no effect on the Kir2 family member1. In accordance, it was shown to inhibit acetylcholine induced K+ currents in mammalian cardiomyocytes2,3.Tertiapin-Q is a derivative of Tertiapin in which Met13 is substituted by a Gln residue. However, unlike native Tertiapin, Tertiapin-Q is non-oxidizable and therefore is more stable4.Tertiapin-Q inhibits the above-mentioned channels with similar affinities and also inhibits Ca2-activated large conductance BK-type K+ channels in a concentration and voltage-dependent manner5.

Supplier :

Alomone Labs

Target :

Kir1.1, Kir3.2 K+ channels

Long Description :

An Inward Rectifier K+ Channel Blocker Conjugated to the Fluorescent Dye ATTO-633

Short Description :

An Inward Rectifier K+ Channel Blocker Conjugated to the Fluorescent Dye ATTO-633

MW :

3159.6 Da

Modifications :

Disulfide bonds between: Cys3-Cys14, Cys5-Cys18 Lys21 - C-terminal amidation ATTO Fluor-633

Effective Concentration :

50 - 200 nM

Activity :

Tertiapin-Q blocks a range of inward rectifier K+ channels (Kir), in particular ROMK1 and GIRK, but with no effect on Kir2 family members1,2. In addition, it was shown to inhibit acetylcholine induced K+ currents in the heart3,4.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

ALCNCNRIIIPHQCWKKCGKK-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage