Scyllatoxin | Alomone Labs STS-370 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Scyllatoxin

catalog :

STS-370

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs Scyllatoxin inhibits rSK2 channels stably expressed in HEK293T cells. - rSK2 channel currents were measured using wholecell voltage clamp in the presence of Ca2+in the pipette solution. A voltage ramp from -120 mV to +60 mV was applied every 10 sec from a holding potential of -80 mV. A. Time course of current amplitude at 0 mV before (black) and during (green) application of 1 nMScyllatoxin(#STS-370). B. Representative current traces at control conditions (black) and following 140 s application of 1 nM Scyllatoxin (green) taken from the experiment in A.

image 2 :

Alomone Labs Scyllatoxin inhibits KCa2.2 channels heterologously expressed inXenopusoocytes. - Oocyte membrane potential was held at +5 mV (in a low Cl-solution) and currents were elicited by intraoocyte CaCl2injection. During the period marked by a green trace (30 seconds) 100 nM ofScyllatoxin(#STS-370) was applied by perfusion.

product information

cat :

STS-370

SKU :

STS-370_0.1 mg

Product Name :

Scyllatoxin

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P16341

Accession Number :

https://www.uniprot.org/uniprotkb/P16341/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Leiurus hebraeus (Hebrew deathstalker scorpion) (Leiurus quinquestriatus hebraeus)

Source :

Synthetic peptide

Gene ID :

KCNN1,KCNN2,KCNN3

Product Page - Scientific background :

Scyllatoxin is a 31 amino acid long toxin, originally isolated from the Leiurus quinquestriatus hebraeus scorpion venom, and is classified as α-KTx 5.1 scorpion toxin family, having three disulfide bridges1,2.Scyllatoxin was shown to compete with 125I-apamin binding in the brain3. Furthermore, Scyllatoxin appears to be selective for apamin-sensitive SK channels. Scyllatoxin inhibits apamin-sensitive SK channel activity in guinea-pig and rabbit hepatocytes4, SK currents in human lymphoblastoma cells5,6, and epinephrine-induced relaxation of visceral smooth muscle7.Scyllatoxin also inhibits the apamin-sensitive after hyperpolarization that follows action potentials in skeletal muscle7 and neurons8. The SK channel-mediated after hyperpolarising current (IAHP) of dorsal vagal neurons, presuming Kca2.3 (SK3), were blocked by Scyllatoxin (20-30 nM)9. HEK 293 cell currents stably expressing hKca2.1 (hSK1) and Kca2.2 (hSK2) were blocked by Scyllatoxin with an IC50 of 80 nM and 287 pM, respectively10.

Supplier :

Alomone Labs

Target :

KCa2 K+ channels

Long Description :

A Potent Blocker of KCa2 (SK) K+ Channels

Short Description :

A Potent Blocker of KCa2 (SK) K+ Channels

MW :

3423 Da

Synonyms :

K+ channel toxin α-KTx 5.1, Leiurotoxin I, LeTx I, ScyTx

Modifications :

Disulfide bonds between: Cys3-Cys21, Cys8-Cys26, and Cys12-Cys28 His31 - C terminal amidation

Molecular formula :

C142H237N45O39S7

Effective Concentration :

20 - 200 nM

Activity :

Scyllatoxin inhibits small conductance Ca2+-activated K+ channels (SK)1-3.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

CAS No :

142948-19-4

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

AFCNLRMCQLSCRSLGLLGKCIGDKCECVKH-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage