Phrixotoxin-1 | Alomone Labs STP-700 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Phrixotoxin-1

catalog :

STP-700

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Ji X, Martin G. BK channels mediate dopamine inhibition of firing in a subpopulation of core nucleus accumbens medium spiny neurons. Brain Res. 2014;1588:1-16 pubmed publisher
Sethi J, Sanchez Alavez M, Tabarean I. Kv4.2 mediates histamine modulation of preoptic neuron activity and body temperature. PLoS ONE. 2011;6:e29134 pubmed publisher
Schaarschmidt G, Wegner F, Schwarz S, Schmidt H, Schwarz J. Characterization of voltage-gated potassium channels in human neural progenitor cells. PLoS ONE. 2009;4:e6168 pubmed publisher

image

image 1 :

Peptide (C)RAPHWYASHMKTR corresponding to amino acid residues 137-149 of mouse prostanoid EP3 receptor (AccessionP34980). 2ndintracellular loop.

image 2 :

Alomone Labs Phrixotoxin-1 inhibits KV4.2 channels expressed in Xenopus oocytes. - A. Time course ofPhrixotoxin-1(#STP-700) action on KV4.2 current amplitude recorded at 10 mV. Current amplitudes were plotted as a function of time. Membrane potential was held at -100 mV and oocytes were stimulated by a 100 ms voltage ramp to +20 mV. 50 nM Phrixotoxin-1 was perfused as indicated by the bar (green) for 220 sec. B. Superimposed examples of KV4.2 channel current in the absence (control) and presence (green) of 50 nM Phrixotoxin-1 (taken from the experiment in A).

product information

cat :

STP-700

SKU :

STP-700_0.1 mg

Product Name :

Phrixotoxin-1

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P61230

Accession Number :

https://www.uniprot.org/uniprotkb/P61230/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Paraphysa scrofa (Chilean copper tarantula) (Phrixotrichus auratus)

Source :

Synthetic peptide

Gene ID :

KCND2,KCND3,SCN9A

Product Page - Scientific background :

Phrixotoxin-1 (PaTx1) is a 29 amino acid peptidyl toxin isolated from the venom of the theraphosid Phrixotrichus auratus, which potently blocks A-type K+ currents.PaTx1 specifically blocks KV4.3 and KV4.2 currents with a 5 nM < IC50 < 70 nM, by modifying the gating properties of these channels.1As with other spider toxins, PaTx1 presents an active molecular surface, including a hydrophobic patch surrounded by charged residues, which are important for their binding on KV channels. Electrophysiological studies show that the PaTx1 blocks KV4 channels via a mechanism similar to that of hanatoxins on KV2 channels, as they bind and stabilize the preferentially closed state of the channel, in a voltage-dependent manner.2PaTx1 is a crucial tool for understanding the contribution of KV4.3 and KV4.2 in cardiac electrogenesis. Treatment of mice with PaTx1 results in numerous transient cardiac adverse reactions including the occurrence of premature ventricular beats, ventricular tachycardia, different degrees of atrioventricular (AV) blockage and prolongation of the QT interval.1

Supplier :

Alomone Labs

Target :

KV4.2, KV4.3 channels

Long Description :

A Potent Blocker of KV4.2 and KV4.3 Channels

Short Description :

A Potent Blocker of KV4.2 and KV4.3 Channels

MW :

3548.4 Da

Synonyms :

κ-theraphotoxin-Ps1a, κ-TRTX-Ps1a, PaTX1

Modifications :

Disulfide bonds between: Cys2-Cys16, Cys9-Cys21, and Cys15-Cys25 Ile29 - C-terminal amidation

Molecular formula :

C156H240N44O37S7.

Effective Concentration :

10 - 100 nM

Activity :

Phrixotoxin-1 specifically and reversibly blocks KV4.2 and KV4.3 channels1.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥99% (HPLC)

CAS No :

221872-97-5

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

YCQKWMWTCDSARKCCEGLVCRLWCKKII-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage