protein

Poneratoxin

STP-360

STP-360_1 mg

Poneratoxin

Non Antibodies

Proteins

P41736

http://uniprot.org/uniprotkb/P41736/entry

Electrophysiology

Lyophilized Powder.

Store the reconstituted solution for the shortest time possible at -20°C. We do not recommend storing the product in working solution for longer than one day. Avoid multiple freeze-thaw cycles.

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. Soluble in DMSO. Prepare a concentrated stock solution by dissolving the lyophilized peptide in DMSO first (e.g., at a concentration between 100-1000x of the final working concentration). Once the peptide is completely dissolved in DMSO, slowly dilute the solution into the working buffer (or water) to the desired final working concentration. Centrifuge all product preparations before use. Avoid multiple freeze-thaw cycles to maintain biological activity. It is recommended to keep the DMSO concentration as low as possible. For cell assays, a final concentration of 0.1%–0.5% DMSO (v/v) is considered safe. For other experiments, a 5% DMSO (v/v) concentration is recommended.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Paraponera clavata (Bullet ant) (Izuela)

Synthetic peptide

SCN8A, SCN9A

Delta-paraponeritoxin-Pc1a (Poneratoxin), originally isolated from the venom of Paraponera clavata (bullet ant), is a 25-amino acid peptide toxin that modulates voltage-gated sodium (NaV) channels, particularly the NaV1.6 and NaV1.7 subtypes1,². The toxin modulates sodium channels by reducing their voltage threshold for activation and inhibiting channel inactivation with EC50 values of 97 ± 10 nM for NaV1.6 and 2.3 ± 0.4 µM for NaV1.7²,3. Structurally, Poneratoxin, along with other ant venom peptides such as Myrmicitoxin1-Pm1a, Myrmicitoxin1-Pm2a, Ectatotoxin-Rm4a and Delta-myrmicitoxin-Ta3a, represents a distinct class of NaV channel modulators. These peptides are structurally and functionally unrelated to previously described classes of peptidic Nav modulators. Their effects on NaV channel currents more closely resemble those of the “site 2” alkaloid toxins, such as batrachotoxin³,⁴. Poneratoxin induces immediate, long-lasting nocifensive behaviors in mice, consistent with its potent modulation of sensory neuron NaV channels. Its activity on NaV1.7, channels expressed predominantly in peripheral sensory neurons, highlights its relevance in pain research and sodium channelopathies3.

Alomone Labs

Nav1.6, Nav1.7 channels

2756.4 Da

Delta-paraponeritoxin-Pc1a, Delta-PPOTX-Pc1a, Pac-TX, PoTx, Pc1a

Arg25 – C-terminal amidation

C129H215N33O31S1

100 nM - 3 µM

Poneratoxin is active on both Nav1.6 and Nav1.7 sodium channels, with a much potent activity on Nav1.6 (EC50=97 nM) than on Nav1.7 (EC50=2.3 µM).

Store the reconstituted solution for the shortest time possible at -20°C. We do not recommend storing the product in working solution for longer than one day. Avoid multiple freeze-thaw cycles.

1-2 Business Days

Israel/IL

≥98% (HPLC)

Lyophilized

Contact Alomone Labs for technical support and product customization

FLPLLILGSLLMTPPVIQAIHDAQR – NH2

Yes

12352202

yes

no