chemical

ProTx-III

STP-150

STP-150_0.1 mg

ProTx-III

Non Antibodies

Proteins

P0DL64

https://www.uniprot.org/uniprotkb/P0DL64/entry

Electrophysiology

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Thrixopelma pruriens (Peruvian green velvet tarantula)

Synthetic peptide

SCN1A,SCN2A,SCN3A,SCN8A,SCN9A

ProTx-III (µ-TRTX-Tp1a) originally isolated from the venom of the peruvian green-velvet tarantula Thrixopelma pruries, is a potent blocker of voltage-gated sodium (NaV) channels, with highest affinity towards NaV1.7 with an IC50 of 11.5 nM, and demonstrates analgesic properties in in vivo models1.NaV channels are responsible for initiating action potentials in electrically excitable cells. They play an important role in the human pain signalling pathway and are important therapeutic targets for treatment of chronic pain. Mutations in NaV channels are also associated with a range of complex pathologic conditions such as epilepsy, myotonic conditions and cardiac arrhythmias1,2.

Alomone Labs

NaV1.7 channels

A Potent Blocker of NaV1.7 Channels

A Potent Blocker of NaV1.7 Channels

3802.5 Da

µ-theraphotoxin-Tp1a, µ-TRTX-Tp1a, Protoxin III, ProTx III

Disulfide bonds between: Cys2-Cys17, Cys9-Cys22, and Cys16-Cys29 Ile 33 - C-terminal amidation

C162H246N52O43S6

1 - 250 nM

ProTx-III is a potent blocker of NaV1.7 voltage-gated Na+ channels1.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

1-2 Business Days

Israel/IL

≥98% (HPLC)

Lyophilized

Contact Alomone Labs for technical support and product customization

DCLKFGWKCNPRNDKCCSGLKCGSNHNWCKLHI-NH2

Yes

12352202

yes

no