MCD peptide | Alomone Labs STM-250 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

MCD peptide

catalog :

STM-250

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone LabsICA-110381enhances KCNQ2/KCNQ3heteromeric channels expressed inXenopusoocytes. - A. Time course of KCNQ2/KCNQ3current enhancement by 0.2 and 1 MICA-110381(#I-160). Currents were elicited by application of voltage step from a holding potential of -100 mV to +60 mV (700 msec). B. Superimposed example traces of current responses before and during perfusion of 0.2 and 1 M ICA-110381 as indicated.

image 2 :

Alomone Labs MCD peptide inhibits KV1.2 channels heterologously expressed inXenopusoocytes. - Time course of KV1.2 current inhibition by 200 nM ofMCD peptide(#STM-250) (orange) compared to natural MCD peptide (green) recorded at -20 mV. The toxins were perfused to the recording bath for a period of 100 sec. Holding potential was -80 mV and test pulses to 60 mV were delivered for 150 msec.

product information

cat :

STM-250

SKU :

STM-250_0.5 mg

Product Name :

MCD peptide

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P01499

Accession Number :

https://www.uniprot.org/uniprotkb/P01499/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Apis mellifera (Honeybee)

Source :

Synthetic peptide

Gene ID :

KCNA1 ,KCNA2

Product Page - Scientific background :

MCD peptide (Mast Cell Degranulating Peptide) is a 22 amino acid peptidyl toxin isolated from Apis mellifera bee venom1. MCD peptide induces histamine release from mast cells2 and release of glutamate in CA1 region of rat hippocampus in dose-dependent manner3.MCD peptide was shown to block KV1.2 channels in Xenopus oocytes4 and to inhibit KV1.1 (IC50 490 nM) and KV1.2 (IC50 440 nM) expressed in cell lines while having no effect on KV1.3, KV1.5 and KV3.15. In accordance, MCD peptide (6.4 µM) enhances the magnitude of field potentials in CA1 region as recorded in hippocampal slices6 and induces epileptiform seizures and brain damage after intracerebroventricular injection of 200 pM in rats7.

Supplier :

Alomone Labs

Target :

KV1.1, KV1.2 K+ channels

Long Description :

A Potent and Selective Blocker of KV1.1 and KV1.2 Channels

Short Description :

A Potent and Selective Blocker of KV1.1 and KV1.2 Channels

MW :

2587 Da

Synonyms :

Mast cell degranulating peptide, MCDP, Peptide 401

Modifications :

Disulfide bonds between: Cys3-Cys15, and Cys5-Cys19 Asn22 - C-terminal amidation

Molecular formula :

C110H192N40O24S4

Effective Concentration :

10 nM - 1 μM

Activity :

MCD peptide inhibits KV1.1 and KV1.2 channels1,2.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

CAS No :

74601-64-2

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

IKCNCKRHVIKPHICRKICGKN-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage