chemical

Jingzhaotoxin-X

STJ-080

STJ-080_0.1 mg

Jingzhaotoxin-X

Non Antibodies

Proteins

P0CH55

https://www.uniprot.org/uniprotkb/P0CH55/entry

Electrophysiology

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Chilobrachys guangxiensis (Chinese earth tiger tarantula) (Chilobrachys jingzhao)

Synthetic peptide

KCND2,KCND3

Jingzhaotoxin-X (JZTX-X) is a 31 amino acid peptidyl toxin originally isolated from the venom of the Chinese earth tiger tarantula, Chilobrachys guangxiensis 1,2. JZTX-X is a selective blocker of voltage-gated potassium (Kv)4.2 and Kv4.3 channels2.Kv channels play key roles in human physiology and pathophysiology. Among the various Kv channels, the Shal-type (Kv4) family is characterized by rapid inactivation and activation, expressing the transient potassium currents within the heart, nervous system, and a subpopulation of smooth muscle cells3. Kv4.2 channel has been identified as a potent factor contributing to the A-type currents within the dorsal horn neurons, and reside at a crucial position for regulating pain processing. Kv4.3 channels are detected within the soma of a neuronal subset of the non-peptidergic nociceptive dorsal root ganglion (DRG), which plays a critical role in the regulation of neuron excitability. Down-regulation of the Kv4.3 channel in pain-sensing neurons can boost pain sensation. JZTX-X toxin was shown to induce long-lasting mechanical hyperalgesia in an animal model2.

Alomone Labs

KV4.2 and KV4.3 Channels

A Blocker of KV4.2 and KV4.3 Channels

A Blocker of KV4.2 and KV4.3 Channels

3706.4 Da

JZTX-X, Jingzhaotoxin F7-15.33

Disulfide bonds between: Cys2-15, Cys9-21 and Cys16-28

C164H242N46O41S6

50 - 500 nM

Jingzhaotoxin-X inhibits the activation of Kv4.2 and Kv4.3 channels and exhibits long-lasting mechanical hyperalgesia in mice1.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

1-2 Business Days

Israel/IL

≥98% (HPLC)

Lyophilized

Contact Alomone Labs for technical support and product customization

LCSREGEFCYKLRKCCAGFYCKAFVLHCYRN-OH

Yes

12352202

yes

no