chemical

Hm1b Toxin

STH-655

STH-655_0.1 mg

Hm1b Toxin

Non Antibodies

Proteins

P0DOC5

https://www.uniprot.org/uniprotkb/P0DOC5/entry

Electrophysiology

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Heteroscodra maculata (Togo starburst tarantula) (Togo starburst baboon spider)

Synthetic peptide

SCN1A, SCN3A

δ-theraphotoxin-Hm1b (Hm1b) is a 34 amino acid peptidyl toxin originally isolated from the venom of the tarantula, Heteroscodra maculata1. Hm1b acts as a potent and highly selective activator of the voltage-gated sodium (NaV) 1.1 and NaV1.3 channels. This toxin inhibits the inactivation of the human NaV1.1 channel (expressed in HEK293 cells) with an EC value of 12 nM1,2.The Hm1b toxin has a high level of sequence similarity to the Hm1a toxin, which is also a selective and specific activator of NaV1.1 channels. Both toxins are members of the extended family of inhibitor cystine knot (ICK) peptides with C1-C4, C2-C5, and C3-C6 disulfide architecture. In addition, they share secondary structure characteristics, specifically an antiparallel β hairpin. Despite the high degree of sequence similarity between Hm1a and Hm1b, the latter is much more stable in biological fluids2.NaV channels are involved in a wide array of physiological processes and play a fundamental role in normal neurological function, especially in the initiation and propagation of action potentials. NaV1.1 channel has been utilized as a therapeutic target for various brain disorders, including epilepsy, Alzheimer's disease, and autisM The NaV1.1 channel also contributes to mechanical pain by regulating the excitability of a specific subset of sensory neurons within the peripheral nervous systeM

Alomone Labs

NaV1.1, Nav1.3, Voltage-gated Na+ channels

An Activator of Nav1.1 and Nav1.3 Channels

An Activator of Nav1.1 and Nav1.3 Channels

3895.4 Da

Delta-theraphotoxin-Hm1b, Delta-TRTX-Hm1b, δ-theraphotoxin-Hm1b

Disulfide bonds between: Cys2-Cys16, Cys9-Cys21 and Cys15-Cys28

C169H241N45O50S6

20 - 500 nM

Hm1b acts as a potent and highly selective activator of NaV1.1 and NaV1.3 voltage-gated sodium channels1.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

1-2 Business Days

Israel/IL

≥98% (HPLC)

Lyophilized

Contact Alomone Labs for technical support and product customization

ECRYLFGGCKTTADCCKHLGCRTDLYYCAWDGTF-OH

Yes

12352202

yes

no