Hm1a Toxin | Alomone Labs STH-601 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Hm1a Toxin

catalog :

STH-601

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Goff K, Goldberg E. Vasoactive intestinal peptide-expressing interneurons are impaired in a mouse model of Dravet syndrome. elife. 2019;8: pubmed publisher

image

image 1 :

Alomone LabsDapoxetine hydrochlorideblocks KV4. 3 channels expressed inXenopusoocytes. - A. Time course of KV4.3 current amplitude and inhibition by 100 MDapoxetine hydrochloride(#D-175). Currents were elicited by application of voltage step from a holding potential of -80 mV to 0 mV (150 msec) every 10 seconds. B. Superimposed example traces of current responses before and during perfusion of 100 M Dapoxetine hydrochloride as indicated.

product information

cat :

STH-601

SKU :

STH-601_0.1 mg

Product Name :

Hm1a Toxin

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P60992

Accession Number :

https://www.uniprot.org/uniprotkb/P60992/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Heteroscodra maculata (Togo starburst tarantula) (Togo starburst baboon spider)

Source :

Synthetic peptide

Gene ID :

KCNB1,KCNB2 ,KCND1,KCND2,KCND3,SCN1A,SCN2A,SCN3A

Product Page - Scientific background :

Hm1a Toxin (δ-TRTX-Hm1a) is a peptide toxin originally isolated from Heteroscodra maculate tarantula venoM The toxin was originally described as a KV2 and KV4 channel blocker1 but has since been found to be a selective and specific activator of NaV1.1 voltage-gated sodium channels2,3. It interacts with extracellular loops connecting transmembrane segments 1-2 and 3-4 in domain IV voltage sensor of the channel to inhibit NaV1.1 fast inactivation2,3. Hm1a inhibits human NaV1.1 channel inactivation expressed in Xenopus oocytes with EC50 value of 38 ± 6 nMNaV1.1 channel is a therapeutic target for brain disorders, such as epilepsy, Alzheimer's disease, and autisM It also contributes to mechanical pain by regulating excitability in a specific subset of sensory neurons within the peripheral nervous systeM

Supplier :

Alomone Labs

Target :

NaV1.1, KV2, KV4 channels

Long Description :

A Selective and Potent Activator of NaV1.1 Channels and Blocker of KV2 and KV4 Channels

Short Description :

A Selective and Potent Activator of NaV1.1 Channels and Blocker of KV2 and KV4 Channels

MW :

3996.4 Da

Synonyms :

Delta-theraphotoxin-Hm1a, Delta-TRTX-Hm1a, Heteroscodratoxin-1, HmTx1, Kappa-theraphotoxin-Hm1a, Kappa-TRTX-Hm1a

Modifications :

Disulfide bonds between: Cys2-Cys16, Cys9-Cys21 and Cys15-Cys28 Ser35 - C-terminal amidation

Molecular formula :

C170H240N48O53S6

Effective Concentration :

100 nM - 1 µM

Activity :

Hm1a is a NaV1.1 specific activator1.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

ECRYLFGGCSSTSDCCKHLSCRSDWKYCAWDGTFS-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

Cited Application :

Electrophysiology

more info or order :

Alomone Labs product webpage