protein

Hm1a-ATTO Fluor 647N

STH-600-FRN

STH-600-FRN_10 mcg

Hm1a-ATTO Fluor 647N

Non Antibodies

Proteins

Electrophysiology, Live cell imaging, Immunofluorescence, Fluorescence staining, Direct flow cytometry

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is lyophilized in 0.5 ml conical vial. The product is soluble in pure water to high-micromolar concentrations (5 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity. Avoid exposure to light.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. Avoid exposure to light.

Heteroscodra maculata (Togo starburst tarantula) (Togo starburst baboon spider)

Modified synthetic peptide

KCNB1,KCNB2 ,KCND1,KCND2,KCND3,SCN1A,SCN2A,SCN3A

Delta-theraphotoxin-Hm1a (Hm1a) is a peptide toxin originally isolated from Heteroscodra maculate tarantula venom. Initially described as a moderate-affinity KV4.1 blocker¹, later studies confirmed it primarily targets NaV1.1 voltage-gated sodium channels²,3. It interacts with extracellular loops connecting transmembrane segments 1-2 and 3-4 in domain IV voltage sensor of the channel to inhibit NaV1.1 fast inactivation2,3. Hm1a inhibits human NaV1.1 channel inactivation expressed in Xenopus oocytes with EC50 value of 38 ± 6 nM. Recent studies show Hm1a restores interneuron firing in Scn1a+/- mice, improving inhibition in Dravet Syndrome models⁴. Electrophysiology confirms it prolongs NaV1.1 activation with minimal effects on NaV1.2 and Na

Alomone Labs

NaV1.1, KV2, KV4 channels

A Fluorescent Conjugate Marker for Sensitive Detection of NaV1.1 Channels

4625 Da

Delta-theraphotoxin-Hm1a, Delta-TRTX-Hm1a, Heteroscodratoxin-1, HmTx1, Kappa-theraphotoxin-Hm1a, Kappa-TRTX-Hm1a

ATTO 647N (conjugated via amine group)

100 nM - 1µM

Hm1a is a NaV1.1 specific activator1.

Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.

1-2 Business Days

Israel/IL

Lyophilized

Contact Alomone Labs for technical support and product customization

ECRYLFGGCSSTSDCCKHLSCRSDWKYCAWDGTFS-OH

Yes

12352202

Yes

no