Hemitoxin | Alomone Labs STH-500 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Hemitoxin

catalog :

STH-500

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs Hemitoxin inhibits KV1.2 channel currents heterologously expressed inXenopusoocytes. - Currents were elicited by application of a 100 msec voltage ramp from a holding potential of -80 mV to 60 mV delivered every 10 seconds. A. Time course of channel activity (current amplitude at -20 mV) before and during application of 500 and 100 nMHemitoxin(#STH-500) as indicated by the bars. B. Example of superimposed current traces before and during application of 500 and 100 nM Hemitoxin (taken from the experiment in A).

product information

cat :

STH-500

SKU :

STH-500_0.1 mg

Product Name :

Hemitoxin

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P85528

Accession Number :

https://www.uniprot.org/uniprotkb/P85528/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Hemiscorpius lepturus (Scorpion)

Source :

Synthetic peptide

Gene ID :

KCNA1 ,KCNA2 ,KCNA3

Product Page - Scientific background :

Hemitoxin is a 35 amino acid peptidyl toxin isolated from the Hemiscorpius lepturus scorpion venom and belongs to the αKTx6 family1. It reversibly blocks KV1.1, KV1.2 and KV1.3 voltage-gated K+ channels with an IC50 of 13 nM, 16 nM and 2 nM, respectively1. It shares 80% sequence similarity with Maurotoxin, and has a unique pairing of its four disulfide bridges1.

Supplier :

Alomone Labs

Target :

KV1.1, KV1.2, KV1.3 K+ channels

Long Description :

A Potent Blocker of KV1.1, KV1.2 and KV1.3 K+ Channels

Short Description :

A Potent Blocker of KV1.1, KV1.2 and KV1.3 K+ Channels

MW :

3897.7 Da

Synonyms :

K+ channel toxin α-KTx 6.15

Modifications :

Disulfide bonds between: Cys3-Cys24, Cys9-Cys29, Cys13-Cys19 and Cys31-Cys34

Molecular formula :

C159H259N49O49S8

Effective Concentration :

1 nM - 1 μM

Activity :

Hemitoxin reversibly inhibits rat voltage-gated K+ channels KV1.1 (KCNA1), KV1.2 (KCNA2) and KV1.3 (KCNA3)1.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

IKCTLSKDCYSPCKKETGCPRAKCINRNCKCYGCS-OH

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage