HsTx1 Toxin | Alomone Labs STH-300 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

HsTx1 Toxin

catalog :

STH-300

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs HsTx-1 Toxin inhibits KV1.3 channels heterologously expressed inXenopusoocytes. - A. Time course ofHsTx1 Toxin(#STH-300) action on KV1.3 currents. Current amplitudes were plotted as a function of time. Membrane potential was held at -80 mV and oocytes were stimulated by a 100 ms voltage ramp to +10 mV. 0.5 nM HsTx1 Toxin was perfused during 160 sec as indicated (green) at 0 mV (70% inhibition) and -12 mV (95% inhibition). B. Superimposed examples of KV1.3 channel current in the absence (black) and presence (green) of 0.5 nM HsTx1 Toxin (taken from the experiment in A).

image 2 :

Alomone LabsTopotecan hydrochlorideblocks 5-HT3A receptors expressed in HEK 293T cells. - 5-HT3A receptor currents were elicited by 10 M 5-HT delivered every 3 minutes.Topotecan hydrochloride(#T-160) was applied 30 seconds before stimulation at 10 100 and 200 M as indicated and completely inhibited the 5-HT induced current in a dose dependent and reversible manner.

product information

cat :

STH-300

SKU :

STH-300_0.1 mg

Product Name :

HsTx1 Toxin

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P59867

Accession Number :

https://www.uniprot.org/uniprotkb/P59867/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Heterometrus spinifer (Asia giant forest scorpion) (Malaysian black scorpion)

Source :

Synthetic peptide

Gene ID :

KCNA1 ,KCNA3,KCNH2,KCNN4

Product Page - Scientific background :

Many K+ channel blockers have been identified in the scorpion venom1. These peptide toxins consist of 29 to 39 amino acids, and commonly share three disulfide bonds. Their 3D structure comprises a helix and a double- or triple-stranded β-sheet2. The functional sites of the toxins are located either on the solvent exposed face of the β-sheet, or on the helix, depending on the type of K+ channel targeted3.Potassium channel toxin alpha-KTx 6.3 (HsTx1) is a 34 amino acid peptidyl toxin isolated from the Heterometrus spinifer (Asia giant forest scorpion, Malaysian black scorpion) venom4. HsTx1 is one of the most active peptidic inhibitors of rat KV1.3 channels ever described, with an IC50 of approx. 12 pM4. HsTX1 belongs to a structural class of scorpion K+ channel inhibitors which includes Pandinus imperator toxin 1 (Pi1) and Maurotoxin (MTX) (#STM-340), two toxins containing 35 residues and four disulphide bridges5.

Supplier :

Alomone Labs

Target :

KV1.3 K+ channels

Long Description :

A Potent Blocker of KV1.3 K+ Channels

Short Description :

A Potent Blocker of KV1.3 K+ Channels

MW :

3818.5 Da

Synonyms :

Potassium channel toxin α-KTx 6.3, Neurotoxin HsTX1

Modifications :

Disulfide bonds between: Cys3-Cys24, Cys9-Cys29, Cys13-Cys31 and Cys19-Cys34 Cys34 - C-terminal amidation

Molecular formula :

C149H246N54O46S9

Effective Concentration :

1 - 100 pM

Activity :

HsTx1 Toxin is a highly potent KV1.3 blocker1.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥99% (HPLC)

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

ASCRTPKDCADPCRKETGCPYGKCMNRKCKCNRC-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage