Huwentoxin-I | Alomone Labs STH-050 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Huwentoxin-I

catalog :

STH-050

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs Azasetronblocks 5-HT3A receptors expressed in HEK 293T cells. - 5-HT3A receptor currents were elicited with 10 M 5-HT delivered every 3 minutes. Azasetron (#A-240) was applied 30 seconds before stimulation at 0.5 1 and 20 nM as indicated and inhibited the 5-HT-induced current in a dose-dependent and reversible manner.

image 2 :

Alomone LabsHuwentoxin-I inhibits NaV1.7 currents heterologously expressed in HEK-293 cells. - A. Time course ofHuwentoxin-I(#STH-050) blocking action on NaV1.7 currents. Maximum current amplitudes were plotted as a function of time. Membrane potential was held at -100 mV and cells were stimulated by a 20 ms voltage step to -30 mV. 200 nM Huwentoxin-I were perfused as indicated by the bar (green) during 350 sec. B. Superimposed examples of NaV1.7 channel current in the absence (control) and presence (green) of 200 nM Huwentoxin-I (taken from the experiment in A).

product information

cat :

STH-050

SKU :

STH-050_0.1 mg

Product Name :

Huwentoxin-I

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P56676

Accession Number :

https://www.uniprot.org/uniprotkb/P56676/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Cyriopagopus schmidti (Chinese bird spider) (Haplopelma schmidti)

Source :

Synthetic peptide

Gene ID :

CACNA1B,SCN1A,SCN2A,SCN3A,SCN4A,SCN8A,SCN9A

Product Page - Scientific background :

Huwentoxin-I (HwTx-I) is a 33-residue peptide toxin that was originally isolated from the venom of the Chinese bird spider (Ornithoctonus huwena). Huwentoxin-I is known to be an inhibitor of tetrodotoxin-sensitive voltage-gated Na+ channels (TTX-S) with IC50 values of ~50 nM and N-type voltage-sensitive Ca2+ channels with IC50 values of ~100 nM in mammalian DRG, hippocampus and insect DUM neurons1. It has only a very weak effect on L-type Ca2+ channels, no effect on TTX-R channels and has virtually no effect on muscle Na+ channels. The selectivity of Huwentoxin-I for Ca2+ channels appears to be higher than ω-conotoxin MVIIA and equivalent to ω-conotoxin GVIA2. Huwentoxin-I demonstrates an antinociceptive effect in the rat model of the formalin test when administrated intrathecally (ED50 ~0.28 µg/kg), without side effects of the ones caused by ω-conotoxin MVIIA3.Huwentoxin-I also blocks neuromuscular transmission by acting on nAChR4.

Supplier :

Alomone Labs

Target :

N-type CaV, TTX-sensitive NaV channels, nAChR

Long Description :

An Antagonist of TTX-Sensitive NaV Channels, N-Type Ca2+ Channels and nAChRs

Short Description :

An Antagonist of TTX-Sensitive NaV Channels, N-Type Ca2+ Channels and nAChRs

MW :

3750 Da

Synonyms :

µ/ω-Theraphotoxin-Hs1a, µ/ω-TRTX-Hh1a, Huwentoxin-1, HwTx-I, Mu/omega-theraphotoxin-Hs1a

Modifications :

Disulfide bonds between: Cys2-Cys17, Cys9-Cys22, and Cys16-Cys29

Molecular formula :

C161H246N48O44S6

Effective Concentration :

50 - 200 nM

Activity :

An inhibitor of TTX-sensitive voltage-gated Na+ channels and N-type voltage-gated Ca2+ channels1. Also found to block neuromuscular transmission2.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

ACKGVFDACTPGKNECCPNRVCSDKHKWCKWKL-OH

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage