GrTx1 | Alomone Labs STG-250 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

GrTx1

catalog :

STG-250

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone LabsGrTx1 inhibits NaV1.7 currents in stably transfected HEK293T cells. - A. Time course ofGrTx1(#STG-250) action on stably transfected NaV1.7 currents. Current amplitudes were plotted as a function of time. Membrane potential was held at -100 mV and currents were elicited by a 100 ms voltage ramp from the holding potential of -100 mV to +60 mV applied every 10 sec using whole-cell voltage clamp. 250 nM GrTx1 were perfused as indicated by the green horizontal bar. B. Superimposed traces of Nav1.7 channel currents in the absence (control) and presence (green) of 250 nM GrTx1 applied for 2.5 min. (taken from the experiment in A).

image 2 :

Alomone LabsICA 121431blocks NaV1. 3 channels expressed inXenopusoocytes. - A. Time course of NaV1.3 current amplitude and inhibition by 0.1 and 1 MICA 121431(#I-170). Currents were elicited by application of a 50 ms voltage step to 0 mV preceded by an inactivation inducing protocol (H.P= -120 mV 1ststep to 0 mV 500 ms recovering at -100 mV for 100 ms followed by the test step) every 10 seconds. B. Superimposed example traces of current responses before and during perfusion of 0.1 and 1 M ICA 121431 as indicated.

product information

cat :

STG-250

SKU :

STG-250_0.1 mg

Product Name :

GrTx1

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P85117

Accession Number :

https://www.uniprot.org/uniprotkb/P85117/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Grammostola rosea (Chilean rose tarantula) (Grammostola spatulata)

Source :

Synthetic peptide

Gene ID :

KCNH2,SCN1A,SCN2A,SCN3A,SCN4A,SCN8A,SCN9A

Product Page - Scientific background :

β-theraphotoxin-Gr1a (GrTx1) is a peptidyl toxin originally isolated from the venom of the ''rosean-tarantula'' (Grammostola rosea). GrTx1 has 29 amino-acid residues, compactly folded by three disulfide bridges with a molecular weight of 3697 Da. Sequence analysis reveals that GrTx1 is closely related to other spider toxins reported to affect various distinct ion channel functions1. The toxin inhibits NaV1.1 (IC50 = 0.63 μM), NaV1.2 (IC50= 0.23 μM), NaV1.3 (IC50 = 0.77 μM), NaV1.4 (IC50= 1.29 μM), NaV1.6 (IC50= 0.63 μM), NaV1.7 (IC50= 0.37 μM) and potassium channels KV11.1 (IC50 = 1.2 μM)2.

Supplier :

Alomone Labs

Target :

NaV, KV11.1 channels

Long Description :

A Blocker of NaV and KV11.1 Channels

Short Description :

A Blocker of NaV and KV11.1 Channels

MW :

3697.5 Da

Synonyms :

β-theraphotoxin-Gr1a, β-TRTX-Gr1a

Modifications :

Disulfide bonds between: Cys2-Cys16, Cys9-Cys21 and Cys15-Cys25

Molecular formula :

C159H243N45O41S8

Effective Concentration :

20 - 500 nM

Activity :

GrTx1 is a blocker of voltage-gated Na+ channels and herg1 channels1.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

YCQKWMWTCDSKRKCCEDMVCQLWCKKRL-OH

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage