protein

GsMTx-4-ATTO Fluor-647N

STG-100-FRN

STG-100-FRN_10 mcg

GsMTx-4-ATTO Fluor-647N

Non Antibodies

Proteins

Electrophysiology, Live cell imaging, Immunofluorescence, Fluorescence staining, Direct flow cytometry

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is lyophilized in 0.5 ml conical vial. The product is soluble in pure water to high-micromolar concentrations (5 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity. Avoid exposure to light.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. Avoid exposure to light.

Grammostola rosea (Chilean rose) tarantula

Modified synthetic peptide

SCN9A, TRPC1, TRPC6, PIEZO1

GsMTx-4 is a 34 amino acid peptidyl toxin originally isolated from the Grammostola rosea (Chilean rose) tarantula venom and belongs to the huwentoxin-1 family1. This toxin inhibits different channels and in addition has antimicrobial activity. It blocks cation-selective mechanosensitive ion channels (strech-activated channels, SACs), without having an effect on whole-cell voltage-sensitive currents1. In addition, it inhibits atrial fibrillation2 as well as the membrane motor of outer hair cells3 at low doses. A medium toxicity on a large spectra of voltage-gated Na+ channels, namely NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.5, NaV1.6 and NaV1.7 was reported. GsMTx-4 also inhibits K+ channels KV11.1 and KV11.2, whereas it does not inhibit K+ channels KV1.1 (IC50 > 85 µM), KV1.4 (IC50 > 85 µM) and KV11.3 (IC50 = 53 µM)4. GsMTx-4 was also found to inhibit both TRPC1 and TRPC6 channels5,6, as well as Piezo1, the mechanosensitive channel7. Antimicrobial activity is shown against several Gram-positive and Gram-negative bacteria as well8.

Alomone Labs

NaV1.7 channels, TRPC1, TRPC6, Piezo1

GsMTx-4-ATTO Fluor-647N (#STG-100-FRN) is a highly pure, natural, and biologically active conjugated peptide toxin. Benefits of GsMTx-4-ATTO Fluor-647N: ✓ Localization and distribution ✓ Clustering and internalization kinetics ✓ Live cell imaging ✓ Single cell detection ✓ Binding kinetics ✓ Direct flow cytometry We gladly take on collaboration projects. Please Contact Us.

4723 Da

M-TRTx-Gr1a, M-theraphotoxin-Gr1a, GsMTx4

Phe34 - C-terminal amidation ATTO Fluor-647NDisulfide bonds between: Cys2-Cys17, Cys9-Cys23, and Cys16-Cys30

0.1 µM – 5 µM

GsMTx-4 is an inhibitor of Na+ voltage-gated channels3 and cation-selective mechanosensitive channels1,2.

Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.

1-2 Business Days

Israel/IL

Lyophilized

Contact Alomone Labs for technical support and product customization

GCLEFWWKCNPNDDKCCRPKLKCSKLFKLCNFSF-NH2

12352202

yes

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