α-Conotoxin PeIA | Alomone Labs STC-970 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

α-Conotoxin PeIA

catalog :

STC-970

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs ?-Conotoxin PeIA inhibits ?3/?2 nAChR heterologously expressed inXenopusoocytes. - A. Time course of?-Conotoxin PeIA(#STC-970) action on ?3/?2 nAChR. Current amplitude were plotted as a function of time. Membrane potential was held at -80 mV and oocytes were stimulated by exposing them to 100 M acetylcholine (Ach) every 100 sec. 50 nM (for 300 sec) 100 nM (for 300 sec) and 200 nM (for 200 sec) ?-Conotoxin PeIA were perfused during the period marked by the bar. B. Superimposed traces of ?3/?2 nAChR channel current in the absence (black) and presence of different concentrations of ?-Conotoxin PeIA (taken from experiment in A).

image 2 :

product information

cat :

STC-970

SKU :

STC-970_0.1 mg

Product Name :

α-Conotoxin PeIA

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

Q1L777

Accession Number :

https://www.uniprot.org/uniprotkb/Q1L777/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Conus pergrandis (Grand cone)

Source :

Synthetic peptide

Gene ID :

CACNA1B,CHRNA10,CHRNA3,CHRNA6,CHRNA9,CHRNB2,CHRNB3,CHRNB4

Product Page - Scientific background :

α-Conotoxin PeIA is a peptide toxin originally isolated from the Conus pergrandis (Grand cone) venom1. This toxin belongs to the α-Conotoxin family which acts on the postsynaptic membrane by binding and inhibiting nicotinic acetylcholine receptors (nAChR)1.It potently and reversibly blocks α9/α10 nAChR (IC50 = 6.9 nM) and α3/β2 (IC50 = 23 nM) and discriminates between rat α9/α10 and α7 nACh receptors1. It also inhibits N-type Ca2+ channels (CaV2.2) (IC50 = 1.1 nM) via the activation of the GABA(B) G-protein coupled receptor in DRG neurons2.

Supplier :

Alomone Labs

Target :

nAChR, CaV2.2 Ca2+ channels

Long Description :

An Antagonist of nAChR and N-Type Ca2+ Channels

Short Description :

An Antagonist of nAChR and N-Type Ca2+ Channels

MW :

1652 Da

Synonyms :

Alpha-conotoxin PeIA

Modifications :

Disulfide bonds between: Cys2-Cys8 and Cys3-Cys16 Cys16 - C-terminal amidation

Molecular formula :

C65H98N22O21S4

Effective Concentration :

20 - 50 nM

Activity :

α-Conotoxin PeIA blocks α9/α10 and α3/β2 nAChR1. Also inhibits N-type Ca2+ channels2.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

CAS No :

866876-88-2

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

GCCSHPACSVNHPELC-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage