α-Conotoxin AuIA | Alomone Labs STC-960 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

α-Conotoxin AuIA

catalog :

STC-960

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs?-Conotoxin AuIA inhibits ?3/?4 nAChR channels heterologously expressed inXenopusoocytes. - Trace of ?3/?4 nAChR channel current in the absence and presence of?-Conotoxin AuIA(#STC-960). Current amplitudes were plotted as a function of time. Membrane potential was held at -80 mV and oocytes were stimulated by exposure to 1 ?M ACh (top bar). ?3/?4 nAChR currents are inhibited by 2 min application of 20 M ?-Conotoxin-AuIA (green bottom bar).

image 2 :

Alomone LabsRamosetron hydrochlorideblocks 5-HT3A receptors expressed in HEK 293T cells. - 5-HT3A receptor currents were elicited by 10M 5-HT delivered every 3 minutes.Ramosetron hydrochloride(#R-145) was applied 30 seconds before stimulation at 0.5 1 and 20 nM as indicated and completely inhibited the 5-HT induced current in a dose dependent manner.

product information

cat :

STC-960

SKU :

STC-960_0.25 mg

Product Name :

α-Conotoxin AuIA

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P56639

Accession Number :

https://www.uniprot.org/uniprotkb/P56639/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Conus aulicus (Princely cone)

Source :

Synthetic peptide

Gene ID :

CHRNA3,CHRNB4

Product Page - Scientific background :

Small peptide toxins of Conus origin are highly useful tools for exploring ligand nicotinic acetylcholine receptors (nAChRs) interactions1. There are three α-conotoxin AuIs found in Conus aulicus: AuIA, AuIB and AuIC. α-Conotoxin AuIA is a 16 amino acid peptidyl toxin, belonging to neuronal α 4/7 subfamily of α-conotoxins. This toxin inhibits mammalian neuronal α3/β4 nicotinic acetylcholine receptor channels expressed in Xenopus oocytes. At relatively low concentrations (0.3 and 1.0 μM), this conotoxin is selective and inhibits only α3/β4 subtypes2.

Supplier :

Alomone Labs

Target :

α3/β4 nAChR

Long Description :

An Antagonist of α3/β4 nAChR

Short Description :

An Antagonist of α3/β4 nAChR

MW :

1726 Da

Synonyms :

Alpha-conotoxin AuIA

Modifications :

Disulfide bonds between: Cys2-Cys8 and Cys3-Cys16 Cys16 - C-terminal amidation

Molecular formula :

C72H96N18O24S4

Effective Concentration :

5 - 20 µM

Activity :

α-Conotoxin AuIA is a blocker of α3/β4 nAChR subtypes1.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

CAS No :

216299-20-6

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

GCCSYPPCFATNSDYC-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage