α-Conotoxin EI | Alomone Labs STC-900 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

α-Conotoxin EI

catalog :

STC-900

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs?-Conotoxin EI inhibits ?1/?1/?/? nAChR channels heterologously expressed inXenopusoocytes. - A. Time course of?-Conotoxin EI(#STC-900) action on muscle ?1/?1/?/? nAChR. Current amplitudes were plotted as a function of time. Membrane potential was held at -80 mV and oocytes were stimulated by exposure to 10 ?M acetylcholine and 3 M PNU-120596 stimulation every 100 seconds. 0.5 M (for 4.5 min green) and 1 M (for 4.5 min red) ?-Conotoxin EI was perfused during the period marked by the bar as indicated. B. Superimposed traces of muscle nAChR channel current in the absence (black) and presence of 0.5 M (green) or 1 M (red) ?-Conotoxin EI (taken from the experiment in A).

product information

cat :

STC-900

SKU :

STC-900_0.1 mg

Product Name :

α-Conotoxin EI

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P50982

Accession Number :

https://www.uniprot.org/uniprotkb/P50982/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Conus ermineus (Agate cone) (Chelyconus ermineus)

Source :

Synthetic peptide

Gene ID :

CHRNA1,CHRNA3,CHRNA4,CHRNA7, CHRNB1,CHRNB2,CHRNB4,CHRND,CHRNG

Product Page - Scientific background :

The nicotinic acetylcholine receptors (nAChRs) are a family of ligand-gated ion channels that are widely expressed throughout the central and peripheral nervous systems. The best characterized nAChR is the receptor at the neuromuscular junction, with four different subunits in a pentameric array, (α1)2β1γδ1.Small peptide toxins of Conus origin known as the Aα-conotoxins are highly useful tools for exploring ligand nAChR interactions. α-Conotoxins are known to be selective and potent competitive antagonists of nicotinic acetylcholine receptors2.α-Conotoxin EI is a 18 amino acid peptidyl toxin, originally isolated from the Conus ermineus (Athlantic fish-hunting cone) venoM In Torpedo nAChRs, α-conotoxin EI selectively binds the agonist site near the α/δ subunit interface. In mammalian nAChRs α-conotoxin EI shows high affinity for both the α/δ and α/γ subunit interfaces (with some preference for the α/δ site)3.The unique binding preference of α-conotoxin EI to the α/δ subunit interface makes it a valuable structural template for superposition of various α-conotoxin possessing distinct receptor subtype specificities4.

Supplier :

Alomone Labs

Target :

α1/β1/γ/δ nAChR

Long Description :

An Antagonist of α1/β1/γ/δ nAChR

Short Description :

An Antagonist of α1/β1/γ/δ nAChR

MW :

2093.4 Da

Synonyms :

Alpha-conotoxin EI

Modifications :

Disulfide bonds between: Cys4-Cys10, Cys5-Cys18 Cys18 - C-terminal amidation X = Hydroxyproline

Molecular formula :

C83H125N27O27S5

Effective Concentration :

500 nM - 1 µM

Activity :

A selective blocker of α1/β1/γ/δ nAChR.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

CAS No :

170663-33-9

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

RDXCCYHPTCNMSNPQIC-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage