chemical

α-Conotoxin PIA

STC-870

STC-870_0.1 mg

α-Conotoxin PIA

Non Antibodies

Proteins

P69658

https://www.uniprot.org/uniprotkb/P69658/entry

Electrophysiology

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Conus purpurascens (Purple cone)

Synthetic peptide

CHRNA6

α-Conotoxin PIA is a peptide toxin originally isolated from Conus purpurascens and belongs to the A superfamily of conotoxins. It is a selective antagonist for heteromeric α6 subunit -containing nicotinic acetylcholine receptors (nAChR). α-Conotoxin PIA binds on the extracellular portion of the nAChR and distinguishes α6 from α3 subunits due to its lower affinity for α3.The alpha-conotoxin PIA has an "omega-shaped" overall topology, containing spacing of Cys residues, disulfide connectivity, and the SNPV (serine, asparagine, proline, valine) sequence in the first peptide loop. The second loop demonstrates a kink in Pro15 that provides a distinct steric and electrostatic environment1-3.Evidence has shown that α-conotoxin PIA has the ability to potently block nicotine-stimulated dopamine release in rat striatal synaptosomes with low nanomolar potency1. Indeed, the IC50 value for α-conotoxin PIA of human chimeric α6/α3/β2/β3 nAChRs is 1.7 nM1.Nicotinic acetylcholine receptors containing α6 subunits in the CNS are involved in a variety of physiological functions, and considered to play an important role in cognitive function, motor activity, pain perception, analgesia and the reinforcing properties of nicotine.They are implicated in the pathophysiology and treatment of diseases like chronic Parkinson's, and Alzheimer's1.

Alomone Labs

α6 nAChR

A Selective nAChRα6 Antagonist

A Selective nAChRα6 Antagonist

1981 Da

Alpha-conotoxin PIA

Disulfide bonds between: Cys4-Cys10 and Cys5-Cys18 Cys18 - C-terminal amidation

C79H125N27O25S4

0.2 - 1 µM

α-Conotoxin PIA is a selective antagonist of α6-containing nAChR1,2.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

1-2 Business Days

Israel/IL

≥98% (HPLC)

669050-68-4

Lyophilized

Contact Alomone Labs for technical support and product customization

RDPCCSNPVCTVHNPQIC-NH2

Yes

12352202

yes

no