Conantokin R | Alomone Labs STC-777 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

Conantokin R

catalog :

STC-777

more info or order :

Alomone Labs product webpage

image

image 1 :

ManufacturerBioassayAlomone Labs Conantokin R efficiently inhibits NMDA receptors expressed in Xenopus oocytes. - Representative NR1A/NR2A current elicited in Mg2+-free bath solution by a continuous co-application of 2 µM glutamate and 10 µM glycine (black bar). Current is significantly inhibited by 3 µM Conantokin R (#STC-777) application (green bar). Membrane potential was held at -80 mV and current was recorded by two-electrode voltage-clamp.

product information

cat :

STC-777

SKU :

STC-777_0.5 mg

Product Name :

Conantokin R

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P58806

Accession Number :

https://www.uniprot.org/uniprotkb/P58806/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Conus radiatus (Rayed cone)

Source :

Synthetic peptide

Gene ID :

GluN2A, GluN2B, GRIN2A, GRIN2B, GRIN2C, GRIN2D

Product Page - Scientific background :

Conantokin R is a peptide toxin originally isolated from the Conus radiatus (Rayed cone) venom, and preferentially antagonizes GluN2A and GluN2B NMDA receptors1,2,4. Conantokins are a group of short linear peptides (17-27 amino acids) originally isolated from the venom of cone snails. They structurally possess a high degree of alpha-helicity in the presence of divalent cations, and contain gamma-carboxyglutamic acid residues. Four naturally occurring conantokins have been identified and characterized to date, Conantokin G, Conantokin T, Conantokin R, and Conantokin-L1,2.Conantokins appear to function by inhibiting the spermine/spermidine stimulation of ion flow through the NMDAR channel and different conantokin species present different NMDA receptor subunit specificity3.NMDR inhibition by conantokins is related to various human pathologies including pain, convulsive disorders, stroke, and Parkinson's disease. The potential pharmacological selectivity of conantokins, coupled to their efficacy in preclinical disease model and favorable safety profiles, indicate that these peptides represent both novel probes for NMDA receptor function as well as an important class of compounds for continued investigation as human therapeutics1.

Supplier :

Alomone Labs

Target :

GluN2B receptor

Long Description :

An Antagonist of GluN2A and GluN2B Receptors

Short Description :

An Antagonist of GluN2A and GluN2B Receptors

MW :

3098 Da

Synonyms :

Con-R

Modifications :

Disulfide bonds between: Cys21-Cys25 X = 4-Carboxyglutamate

Molecular formula :

C127H201N35O49S3

Effective Concentration :

50 - 500 nM

Activity :

Conantokin R is a potent and non-competitive NMDA receptor 2A/B antagonist1.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

CAS No :

202925-60-8

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

GEXXVAKMAAXLARXNIAKGCKVNCYP-OH

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage