chemical

ω-Conotoxin CVIE

STC-760

STC-760_0.1 mg

ω-Conotoxin CVIE

Non Antibodies

Proteins

Q9N633

https://www.uniprot.org/uniprotkb/Q9N633/entry

Electrophysiology

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Conus catus (Cat cone)

Synthetic peptide

CACNA1B

ω-Conotoxin CVIE is a peptide toxin originally isolated from the sea snail-cat cone (Conus Catus). It is a selective blocker of N-type CaV channels and has an IC50 of 2.6 nM1. It is hypothesized that CVIE mostly interacts with the extracellular part of the channels but also modulates calcium channel binding kinetics and affinity through interaction with the channel's intracellular part and auxiliary subunits. CVIE has a stronger affinity for inactivated calcium channels. Calcium channels recover from CVIE in a voltage dependent manner; recovery is faster at strong membrane hyperpolarization. Since voltage gated calcium channels play an important role in regulating neuronal excitability and nociceptors signal transduction, CVIE has been found to be effective in the alleviation of neuropathic and inflammatory pain2. Unlike other substances that alleviate pain CVIE does not produce sedation and motor-deficit side effect in vitro but further investigation is required for in vivo data3.

Alomone Labs

N-Type Ca2+ channels

A Blocker of N-Type Ca2+ Channels

A Blocker of N-Type Ca2+ Channels

2639 Da

Omega conotoxin-CVIE, Omega conotoxin-CVIE-2

Disulfide bonds between: Cys1-Cys16, Cys8-Cys20 and Cys15-Cys25 Cys25 - C-terminal amidation

C98H164N40O34S6

1-10 nM

ω-Conotoxin CVIE is a selective voltage-gated N-type Ca2+ channel inhibitor1-3.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

1-2 Business Days

Israel/IL

≥98% (HPLC)

Lyophilized

Contact Alomone Labs for technical support and product customization

CKGKGASCRRTSYDCCTGSCRSGRC-NH2

Yes

12352202

yes

no