chemical

ω-Conotoxin CVIA

STC-750

STC-750_0.1 mg

ω-Conotoxin CVIA

Non Antibodies

Proteins

P58917

https://www.uniprot.org/uniprotkb/P58917/entry

Electrophysiology

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Conus catus (Cat cone)

Synthetic peptide

CACNA1B

CaV channels mediate Ca2+ influx through cell membrane as a response to membrane depolarization. N-type channels require strong depolarization for activation and are mostly expressed in neurons where they initiate neurotransmission in fast synapses and mediate Ca2+ entry into cell bodies and dendrites1.ω-conotoxin CVIA is a 27 amino acid neuropeptide2 belonging to a class of four new ω-conotoxins (CVIA-D) extracted from the venom of the Conus Catus snail. These peptides inhibit neuronal voltage sensitive Ca2+ channels in mammals. Interestingly, unlike other ω-conotoxins CVID has a loop 4 sequence and has the highest affinity to N-type channels over other types of Ca2+ channels while CVIA shows a lower affinity to N-type channels.CVIA is efficient in the management of severe pain, has clinical potential in ischemic brain injury and was also found to inhibit the contraction of electrically stimulated rat vas deferens with an IC50 of 205 nM3.

Alomone Labs

CaV2.2 channels

A Blocker of N-Type CaV Channels

A Blocker of N-Type CaV Channels

2642 Da

Omega-conotoxin CVIA

Disulfide bonds between: Cys1-Cys16, Cys8-Cys20 and Cys15-Cys25 C25 - C-terminal amidation

C97H161N39O36S6

50 - 200 nM

ω-Conotoxin CVIA is an N-type Ca2+ channel blocker1.

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

1-2 Business Days

Israel/IL

≥99% (HPLC)

Lyophilized

Contact Alomone Labs for technical support and product customization

CKSTGASCRRTSYDCCTGSCRSGRC-NH2

Yes

12352202

yes

no