µ-Conotoxin BuIIIC | Alomone Labs STC-690 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

µ-Conotoxin BuIIIC

catalog :

STC-690

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone LabsGinkgolideB blocks 5-HT3A receptors expressed in HEK 293T cells. - 5-HT3A receptor currents were elicited by 10 M 5-HT delivered every 3 minutes.GinkgolideB(#G-170) was applied 30 seconds before stimulation at 50 200 and 500 M as indicated and inhibited the 5-HT induced current in a dose dependent and reversible manner.

image 2 :

Alomone Labs -Conotoxin BuIIIC blocks NaV1.4 currents expressed in Xenopus oocytes. - A. Time course of-Conotoxin BuIIIC(#STC-690) blocking action on NaV1.4 currents. Peak current amplitude was plotted as a function of time. Membrane potential was held at -100 mV and cells were stimulated by a 100 ms voltage step to -10 mV. 20 nM -Conotoxin BuIIIC were perfused as indicated by the bar (green) during 5 min. B. Superimposed examples of NaV1.4 channel current in the absence (control) and presence (green) of 20 nM -Conotoxin BuIIIC (taken from the experiment in A).

product information

cat :

STC-690

SKU :

STC-690_0.1 mg

Product Name :

µ-Conotoxin BuIIIC

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

C1J5M7

Accession Number :

https://www.uniprot.org/uniprotkb/C1J5M7/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Conus bullatus (Bubble cone)

Source :

Synthetic peptide

Gene ID :

SCN4A

Product Page - Scientific background :

μ-conotoxin BuIIIC, is a peptide toxin originally isolated from Conus Bullatus (Bubble cone). It has the characteristic arrangement of six-cysteine residues (-CC-C-C-CC-) and comprises 26 amino acid residues. μ-conotoxin BuIIIC was demonstrated to be a potent irreversible inhibitor of the voltage-gated Na+ channel skeletal muscle subtype NaV1.4, expressed in Xenopus oocytes. 1 μM toxin was shown to block 96% of the channel. The novel structural determinants of BuIIIC along with its ability to potently inhibit NaV1.4 make this conotoxin useful in defining features of the NaV1.4 pharmacophore and thereby facilitate the design of highly subtype-specific ligands that target NaV1.41.

Supplier :

Alomone Labs

Target :

NaV1.4 Na+ channels

Long Description :

A Blocker of NaV1.4 Channel

Short Description :

A Blocker of NaV1.4 Channel

MW :

3006 Da

Synonyms :

Mu-Conotoxin BuIIIC

Modifications :

Disulfide bonds between: Cys5-Cys19, Cys6-Cys25, and Cys15-Cys26 Cys26 - C-terminal amidation

Molecular formula :

C116H189N49O34S6

Effective Concentration :

1 pM - 20 nM

Activity :

µ-Conotoxin BuIIIC is a selective NaV1.4 blocker1.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

IVDRCCNKGNGKRGCSRWCRDHSRCC-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage