µ-Conotoxin BuIIIB | Alomone Labs STC-661 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

µ-Conotoxin BuIIIB

catalog :

STC-661

more info or order :

Alomone Labs product webpage

image

image 1 :

Alomone Labs -Conotoxin BuIIIB inhibits NaV1.4 currents heterologously expressed in Xenopus oocytes. - A. Time course of-Conotoxin BuIIIB(#STC-661) blocking action on NaV1.4 peak current amplitude. Maximum current amplitudes were plotted as a function of time. Membrane potential was held at -100 mV and cells were stimulated by a 100 ms voltage step to -20 mV. 20 nM -Conotoxin BuIIIB were perfused as indicated by the bar (green) during 150 sec. B. Superimposed examples of NaV1.4 channel current in the absence (control) and presence (green) of 20 nM -Conotoxin BuIIIB (taken from the experiment in A).

product information

cat :

STC-661

SKU :

STC-661_0.1 mg

Product Name :

µ-Conotoxin BuIIIB

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

C1J5M6

Accession Number :

https://www.uniprot.org/uniprotkb/C1J5M6/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Conus bullatus (Bubble cone)

Source :

Synthetic peptide

Gene ID :

SCN1A,SCN2A,SCN3A,SCN4A

Product Page - Scientific background :

μ-Conotoxin BuIIIB, is a peptide toxin originally isolated from Conus Bullatus (Bubble cone). It is characterized by a six-cysteine frame-work (-CC-C-C-CC-) cross-linked by three disulfide bridges. μ-Conotoxin BuIIIB was demonstrated to be a potent irreversible inhibitor of the NaV skeletal muscle subtype NaV1.4 heterologously expressed in Xenopus oocytes. 1 μM toxin was shown to block 96% of the channel1. In addition, μ-conotoxin BuIIIB potently blocks NaV1.3 expressed in oocytes, with an IC50 of 0.1 μM NaV1.3 is involved in pain perception; therefore μ-conotoxin BuIIIB should serve as a valuable tool to evaluate the contribution of the NaV1.3 subtype to various pain states2,3.

Supplier :

Alomone Labs

Target :

NaV1.2, NaV1.3 and NaV1.4 Na+ channels

Long Description :

A Blocker of NaV1.4 and NaV1.3 and NaV1.2 Na+ Channels

Short Description :

A Blocker of NaV1.4 and NaV1.3 and NaV1.2 Na+ Channels

MW :

2763 Da

Synonyms :

Mu-Conotoxin BuIIIB

Modifications :

Disulfide bonds between: Cys5-Cys17, Cys6-Cys23, and Cys13-Cys24 Cys24 - C-terminal amidation

Molecular formula :

C106H172N46O30S6

Effective Concentration :

1 - 20 nM

Activity :

µ-Conotoxin BuIIIB is a selective NaV1.2, NaV1.3 and NaV1.4 blocker1.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

VGERCCKNGKRGCGRWCRDHSRCC-NH2

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage