κ-Conotoxin RIIIJ | Alomone Labs STC-660 product information

product summary

company name :

Alomone Labs

product type :

chemical

product name :

κ-Conotoxin RIIIJ

catalog :

STC-660

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Smith K, Browne L, Selwood D, McAlpine D, Jagger D. Phosphoinositide Modulation of Heteromeric Kv1 Channels Adjusts Output of Spiral Ganglion Neurons from Hearing Mice. J Neurosci. 2015;35:11221-32 pubmed publisher

image

image 1 :

Alomone Labs MCI-225 blocks 5-HT3A receptors expressed in HEK 293T cells. - 5-HT3A receptor currents were elicited by 10 M 5-HT delivered every 3 minutes.MCI-225(#M-190) was applied 30 seconds before stimulation at 10 and 100 nM as indicated and inhibited the 5-HT induced current in a dose-dependent and reversible manner.

image 2 :

Alomone Labs ?-Conotoxin RIIIJ inhibits KV1.2 currents in Xenopus oocytes. - A. Time course of?-Conotoxin-RIIIJ(#STC-660) action on Kv1.2 currents. Current amplitudes were plotted as a function of time. Membrane potential was held at ?80 mV and oocytes were stimulated by a 100 ms voltage ramp to 0 mV. 50 nM ?-Conotoxin-RIIIJ were perfused as indicated by the bar (green) at -15 mV for 160 sec. B. Superimposed examples of KV1.2 channel current in the absence (control) and presence of 50 nM ?-Conotoxin-RIIIJ (taken from the experiment in A).

product information

cat :

STC-660

SKU :

STC-660_0.1 mg

Product Name :

κ-Conotoxin RIIIJ

Group Type :

Non Antibodies

Product Type :

Proteins

Accession :

P0CG45

Accession Number :

https://www.uniprot.org/uniprotkb/P0CG45/entry

Applications :

Electrophysiology

Formulation :

Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reconstitution and Solubility :

Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Solubility :

Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 µM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.

Storage Before Reconstitution :

The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.

Origin :

Conus radiatus (Rayed cone)

Source :

Synthetic peptide

Gene ID :

KCNA2

Product Page - Scientific background :

κ-conotoxin RIIIJ (κ-M-RIIIJ), is a peptide toxin belonging to the conotoxin M superfamily, and was originally isolated from Conus radiatus (Rayed cone) venoM This conopeptide inhibits the homotetrameric human KV1.2 channels (IC50 = 33 nM)3. κ-M-RIIIJ was found to exert cardioprotective effects, presumably due to the high affinity of this peptide for mammalian KV1.2 and KV1.2- containing heteromultimers3.Voltage-gated K+ channels represent a structurally and functionally diverse group of membrane proteins. These channels establish the resting membrane potential and modulate the frequency and duration of action potentials in nerve and muscle1,2.

Supplier :

Alomone Labs

Target :

KV1.2 K+ channels

Long Description :

Voltage-Gated K+ Channel Blocker

Short Description :

Voltage-Gated K+ Channel Blocker

MW :

2807 Da

Synonyms :

κ-M-RIIIJ, Kappa-conotoxin RIIIJ

Modifications :

Disulfide bonds between: Cys4-Cys17, Cys5-Cys22 and Cys12-Cys23 X = Hydroxyproline

Molecular formula :

C117H184N32O36S6

Effective Concentration :

30 nM

Activity :

κ-Conotoxin RIIIJ is a blocker of KV1.2 channels1.

Storage of solutions :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Purity :

≥98% (HPLC)

Form :

Lyophilized

Comment :

Contact Alomone Labs for technical support and product customization

Sequence :

LXXCCTXXKKHCXAXACKYKXCCKS-OH

Is Toxin :

Yes

UNSPSC :

12352202

Bioassay Tested :

yes

Steril endotoxin free :

more info or order :

Alomone Labs product webpage